Amino acids cannot be rapidly utilized by microorganisms as a gradually metabolized carbon source.L-serine dehydratase (L-SerDH) catalyzes the one-step conversion of L-serine into pyruvate and ammonia, facilitating central carbon metabolism to generate biomass without the consumption of ATP and reducing power.L-serine is a key intermediate in the formate utilization pathway (reductive glycine pathway).Therefore, improving the utilization of L-serine can provide valuable insights into the utilization of formate and serine as carbon sources.This paper explored serine tolerance of Pichia pastoris.Results demonstrated that P.pastoris exhibited tolerance to 20 g/L of L-serine.Expression optimization of endogenous L-serine dehydratase was carried out in P.pastoris.Eight heterologous L-serine dehydratases were expressed separately in glycine auxotroph P.pastoris.The results indicated that L-SerDH encoded by the E.coli tdcG gene had the best effect on the improvement of serine utilization, with the final OD600 increased to 1.6-fold that of the original strain.This study represents the validation of L-serine tolerance in P.pastoris and screens a superior source of L-serine dehydratase.These findings offer valuable insights into enhancing enzyme sources for serine utilization in P.pastoris.
LIU Luyao
,
LI Kang
,
WANG Shijie
,
BAI Zhonghu
,
YANG Yankun
,
JIN Jian
. Expression of Escherichia coli L-serine dehydratase improves growth of glycine auxotroph Pichia pastoris on serine[J]. Food and Fermentation Industries, 2024
, 50(13)
: 16
-24
.
DOI: 10.13995/j.cnki.11-1802/ts.039062
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