Comparative analysis of milk composition and lactation milk metabolism groups in sheep and goats

  • WANG Chunwei ,
  • XU Quanzhong ,
  • WANG Lequn ,
  • FENG Shuang ,
  • FENG Rui ,
  • ZHENG Zhong ,
  • ZHANG Li ,
  • ZHANG Liguo ,
  • SU Xiaohu
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  • 1(The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Inner Mongolia Autonomous Region, Hohhot 010020, China)
    2(Ulanqab Animal Husbandry Workstation, Ulanqab Agriculture and Animal Husbandry Bureau, Inner Mongolia Autonomous Region, Ulanqab 012000, China)

Received date: 2023-06-01

  Revised date: 2023-07-04

  Online published: 2024-08-02

Abstract

A comparative analysis of milk composition and milk metabolome between sheep and goats in mid-lactation was conducted to help resolve the mechanisms of differences in lactation traits between the two species and to provide a reference for the selection and breeding of dairy sheep.Six sheep and goat milk samples were collected separately on the 90th day after parturition, and the milk routine composition was analyzed by milk composition analyzer for both species, and the metabolites in milk were analyzed by liquid chromatography coupled with mass spectrometry using a non-targeted metabolomics approach.Results showed that milk fat, lactose, protein, casein, solids, and non-milk fat solids were significantly higher in sheep milk than in goat milk (P<0.05).Milk metabolome analysis revealed that 1 513 metabolites were detected, of which 455 were annotated and 292 differential metabolites were identified based on conditional screening.Pathway enrichment analysis of the differential metabolites revealed that a total of 53 functional pathways were enriched, among which 30 and 23 functional pathways were enriched for the dominant metabolites in sheep and goat milk, respectively, and the most enriched pathway for the dominant metabolites in sheep milk was the oxidation of branched-chain fatty acids, whereas in goat milk it was the pentasaccharide phosphate pathway.The results of the study provide some help for the analysis of the regulatory mechanism of lactation traits in dairy livestock and provide some reference for the improvement of the feeding and lactation performance of dairy sheep.

Cite this article

WANG Chunwei , XU Quanzhong , WANG Lequn , FENG Shuang , FENG Rui , ZHENG Zhong , ZHANG Li , ZHANG Liguo , SU Xiaohu . Comparative analysis of milk composition and lactation milk metabolism groups in sheep and goats[J]. Food and Fermentation Industries, 2024 , 50(13) : 140 -148 . DOI: 10.13995/j.cnki.11-1802/ts.036324

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