Food and Fermentation Industries >

2024 , Vol. 50 >Issue 14: 1 - 9

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039106

Efficient production of recombinant collagen in Corynebacterium glutamicum by expression elements optimization

  • CHENG Yifan ,
  • ZHANG Meng ,
  • XU Fei
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  • (Key Lab of Ministry of Education of Industrial Biotechnology, School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2024-03-06

  Revised date: 2024-04-10

  Online published: 2024-08-02

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Abstract

Recombinant collagen is a biopolymer with broad potential applications in various fields, such as biomedicine and tissue engineering.The triple-helix structure of collagen imparts unique biological functions and biocompatibilit but also increases the complexity of its expression in microbial systems.In this study, bacterial collagen V-B was used as a model protein to promote the expression of recombinant collagen in Corynebacterium glutamicum through optimization of expression elements.Firstly, the most efficient promoter tac-R0 was obtained through promoter screening and fermentation duration optimization.Subsequently, the RBS calculator was used to design a mutation library for ribosome binding sites (RBS) and aligned spacing (AS).The RBS and AS with the highest strength were identified, which increased the yield of V-B to 514 mg/L.Furthermore, through the tandem combination of multi-gene expression cassettes, the final yield of V-B increased by 8.4-fold compared to the initial level, reaching 697 mg/L.This study lays a foundation for the industrial production of recombinant collagen.

Key words： recombinant collagen; Corynebacterium glutamicum; promoter; ribosome binding sites; multi-gene expression cassettes

Cite this article

CHENG Yifan , ZHANG Meng , XU Fei . Efficient production of recombinant collagen in Corynebacterium glutamicum by expression elements optimization[J]. Food and Fermentation Industries, 2024 , 50(14) : 1 -9 . DOI: 10.13995/j.cnki.11-1802/ts.039106

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