Food and Fermentation Industries >

2024 , Vol. 50 >Issue 14: 270 - 278

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038678

High-performance liquid chromatography-tandem quadrupole-time of flight-mass spectrometry for determination of 29 pesticides in litchi

  • WANG Siwei ,
  • LIU Yanping ,
  • DING Xiaobo ,
  • LI Jingming
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  • 1(Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China)
    2(Luzhou Academy of Agricultural Sciences, Luzhou 646000, China)

Received date: 2024-01-24

  Revised date: 2024-04-10

  Online published: 2024-08-02

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Abstract

An analytical method was established for the simultaneous determination of 29 pesticides in litchi by a high-performance liquid chromatography-tandem quadrupole-time of flight-mass spectrometry (HPLC-Q-TOF/MS) technique combined with an optimized QuEChERS procedure.The samples were extracted by acetonitrile and the acetonitrile supernatant was collected for purification after salting-out (magnesium sulfate anhydrous and sodium chloride) and centrifugation.Under the optimized chromatographic and mass spectrometric conditions, the data were collected by electrospray ionization (ESI) sources in positive and negative ion modes.The calibration curves of 29 pesticides showed good linearity in a concentration range of 0.001-0.2 mg/L (R2>0.99).The average recoveries were 70.2%-102.4% at three spiked levels (0.01, 0.1, 0.50 mg/kg), while the relative standard deviations (RSD) were 2.4%-6.2%.The limits of detection and limits of quantification ranged from 0.1 μg/kg to 3 μg/kg and 0.3 μg/kg to 10 μg/kg, respectively.This method was a simple and efficient method with high sensitivity and precision and had practical value for the rapid detection of residues of 29 pesticides in litchi.

Key words： litchi; 29 pesticides; high performance liquid chromatography-tandem quadrupole-time-of-flight-mass spectrometry

Cite this article

WANG Siwei , LIU Yanping , DING Xiaobo , LI Jingming . High-performance liquid chromatography-tandem quadrupole-time of flight-mass spectrometry for determination of 29 pesticides in litchi[J]. Food and Fermentation Industries, 2024 , 50(14) : 270 -278 . DOI: 10.13995/j.cnki.11-1802/ts.038678

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