Carnosine is a new type of food supplement with various bioactive functions, which has been widely used in fields such as health products, functional beverages, cosmetology, etc.However, there are few studies on the research and application of carnosine in the repair of liver injury.This study aimed to verify the repair effect of carnosine on liver injury and explore its mechanism.L-02 cells were pretreated with carnosine for 12 hours and then were treated with 400 mmol/ml tert butyl hydrogen peroxide (TBHP) for 4 hours.The cell viability was assessed using the cell count kit-8 (CCK-8), the levels of alanine transaminase (ALT), aspartate transaminase (AST), reactive oxygen species (ROS), and glutathione (GSH) were detected using their respective activity assay kits, the cell apoptosis was measured by flow cytometry, the expression of Keap1-Nrf2 and JNK-Cas-3 signaling pathway-related proteins were quantified by western blot.Results showed that after carnosine pretreatment, the activities of ALT and AST were significantly reduced in the L-02 cell culture medium, the formation of ROS and cell apoptosis were significantly inhibited, and the contents of GSH were significantly increased.The protein expression of nuclear factor-erythroid 2-related factor-2(Nrf2), quinone oxidoreductase 1(NQO1), heme oxygenase-1(HO-1), and B-cell lymphoma 2(Bcl-2) were upregulated by anserine, with the downregulation of Kelch like ECH associated protein 1(Keap1), amino-terminal kinases phosphorylation(p-JNK), and caspase-3(Cas-3).Carnosine effectively alleviated TBHP-induced hepatocyte injury by regulating the Keap1-Nrf2 and JNK-Cas-3 signaling pathways.
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