Food and Fermentation Industries >

2024 , Vol. 50 >Issue 16: 30 - 39

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039631

Screening and optimization of chemically defined medium for alkaline protease production by Bacillus licheniformis

  • YAO Qiaoer ,
  • ZHANG Ying ,
  • WANG Yonghong
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  • (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China)

Received date: 2024-04-19

  Revised date: 2024-05-09

  Online published: 2024-09-19

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Abstract

In this study, a systematic screening and optimization approach was employed to identify and characterize the optimal chemically defined medium for cultivating Bacillus licheniformis to produce alkaline protease.The initial chemically defined medium CDM0 was constructed based on the analysis of the composition of soybean meal and relevant literature reviews.The components that significantly affected the growth of the bacterium (growth reduced by more than 20%) were preliminarily identified through single component removal experiments, including three bases, four vitamins, 11 amino acids, as well as various metal ions and inorganic salts.Based on this, the components that were taken out of CDM0 were added respectively and then five key nutrients with a more than 20% enhancement in cell growth were screened out.Then the optimal combination of (NH4)2SO4, adenine, and asparagine was obtained from a total of 26 combinations of the five key nutrients.Furthermore, the effects of glycerol, five monosaccharides, and three disaccharides on bacterial growth and alkaline protease chemically defined were also investigated.Ultimately, an optimized chemical complete synthesis medium, CDM3, was developed with galactose as the carbon source.The experimental results demonstrated that the cell growth and alkaline protease production under CDM3 were found to be significantly increased compared to the initial medium CDM0, where the enzyme viability was elevated by 2.5-fold to 10 300.7 U/mL.The findings of this experiment provide a foundation for future research on the metabolic characteristics of this strain.

Key words： Bacillus licheniformis; alkaline protease; chemically defined medium; screening and optimization; carbon source

Cite this article

YAO Qiaoer , ZHANG Ying , WANG Yonghong . Screening and optimization of chemically defined medium for alkaline protease production by Bacillus licheniformis[J]. Food and Fermentation Industries, 2024 , 50(16) : 30 -39 . DOI: 10.13995/j.cnki.11-1802/ts.039631

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