Stachyose, as a natural prebiotic, can promote the proliferation of lactic acid bacteria, but the metabolic pathway of stachyose in lactic acid bacteria is still completely resolved.This study attempts to establish a high-performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD) method for the detection of stachyose and its metabolites.The dynamic changes of stachyose and its metabolites were intended to provide technical support for the interpretation of the metabolic pathway of stachyose in lactic acid bacteria.Results showed that using Waters BEH Amide chromatographic column combined with ELSD detector, the elution conditions were acetonitrile (80%)∶water (20%), the flow rate of mobile phase was 1.0 mL/min, the temperature of ELSD detector was 60 ℃, and the flow rate of carrier gas was 1.0 mL/min.Stachyose and its metabolites can be well separated, and the detection reproducibility is good and the stability is high, indicating that this method can be used for the detection of stachyose and its metabolites.
JIN Yuying
,
ZHONG Xianfeng
,
XU Jiahao
,
ZHENG Jianwei
,
WANG Ali
,
CHEN Ziqi
,
REN Hong
,
CHEN Xin
,
LU Huaiyu
,
HUANG Guidong
. Determination of stachyose and its metabolites by high-performance liquid chromatography-evaporative light-scattering detector[J]. Food and Fermentation Industries, 2024
, 50(16)
: 293
-298
.
DOI: 10.13995/j.cnki.11-1802/ts.038139
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