Food and Fermentation Industries >

2024 , Vol. 50 >Issue 16: 332 - 340

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036240

Study on mechanism of Haematococcus pluvialis extract in skin age-delaying based on UPLC-MS/MS and network pharmacology

  • TANG Rong ,
  • SUN Jinyue ,
  • LI Junxiang ,
  • HE Congfen
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  • 1(College of Chemistry and Materials Science, Beijing Technology and Business University, Beijing 100048, China)
    2(AGECODE R&D CENTER Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314006, China)
    3(Harvest Biotech Co.Ltd., Jiaxing 314006, China)

Received date: 2023-05-24

  Revised date: 2023-06-30

  Online published: 2024-09-19

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Abstract

This study aimed to identify the active ingredient of Haematococcus pluvialis extract (HPE) by UPLC-MS/MS, to predict the mechanism of HPE in delaying skin aging by network pharmacology and to verify the mechanism experimentally.Based on TCMSP, SwissTargetPrediction, CellAge, and other databases that collect the intersection targets of HPE and aging, the protein-protein interaction networks were used to screen the core targets and key components, and gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed on the core targets.Results showed that there were 16 active ingredients of HPE, corresponding to 196 targets, and the protein-protein interaction networks screened 30 core targets, corresponding to 14 key ingredients.GO enrichment analysis and KEGG pathway enrichment analysis revealed the regulatory activity of HPE to inflammation through mitogen-activated protein kinase signaling, transient receptor potential ion channel mediator regulation, nuclear factor kappa-B signaling, etc.Molecular docking was used to predict the binding ability of key components to the core targets, and the docking scores showed good binding ability between the key components and the core targets.Furthermore, in vitro experiments showed that HPE could significantly inhibit the expression of inflammatory factors in mouse mononuclear macrophage cells and increase the collagenⅠlevel of human foreskin fibroblast cells upon ultraviolet A irradiation.HPE may delay skin aging through the regulation of inflammatory pathways and inflammatory factors and have multi-component-multi-target-multi-pathway characteristics.

Key words： Haematococcus pluvialis extract; UPLC-MS/MS; network pharmacology; skin aging

Cite this article

TANG Rong , SUN Jinyue , LI Junxiang , HE Congfen . Study on mechanism of Haematococcus pluvialis extract in skin age-delaying based on UPLC-MS/MS and network pharmacology[J]. Food and Fermentation Industries, 2024 , 50(16) : 332 -340 . DOI: 10.13995/j.cnki.11-1802/ts.036240

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