Food and Fermentation Industries >

2024 , Vol. 50 >Issue 17: 23 - 30

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037408

Treatment of food wastewater using immobilized heterotrophic Chlorella vulgaris by Aspergillus niger pellets

  • YANG Chan ,
  • ZHENG Xiao ,
  • CONG Wenjie ,
  • GULTOM Sarman Oktovianus ,
  • WANG Mingxuan ,
  • ZHOU Hualan ,
  • LI Zhenhai ,
  • WANG Jiguo ,
  • ZHANG Jianguo
Expand
  • 1(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)
    2(Department of Agricultural and Biosystems Engineering,Papua University Manokwari,Papua Barat 98315,Indonesia)
    3(Toroivd Technology Company Limited, Shanghai 200439, China)

Received date: 2023-09-18

  Revised date: 2024-03-10

  Online published: 2024-10-10

Fold

Abstract

Food wastewater was rich in proteins, carbohydrates, fat, urea, ammonia nitrogen, phosphorus and other nutrients, which made it difficult and high cost to be treated. Aspergillus niger pellets had advantages of high biological activity, high settling rate and easy separation from liquid phase.Chlorella vulgaris had advantages of high growth rate and efficient biomass production.Therefore, the treatment of food wastewater using immobilized C.vulgaris on A.niger pellets was a low-cost, high-efficient, pollution-free and sustainable approach.In this research, this approach was developed through media optimization for A.niger pellets formation, and co-cultivation conditions of A.niger and C.vulgaris.All three media, CCM medium, A medium and modified BG-11 medium, were suitable for pellets formation.A.niger pellet was better than A.niger spore for C.vulgaris harvest according to results.After optimization, 90.25% COD, 43.53% TN, 94.2%TP of soybean wastewater was removed.Therefore, this approach of immobilized algae on fungal pellets was efficient to remove nutrients from food wastewater, and proved a solid base for its further application.

Key words： Aspergillus niger; Chlorella vulgaris; mycelium pellets; food wastewater; removal ratio

Cite this article

YANG Chan , ZHENG Xiao , CONG Wenjie , GULTOM Sarman Oktovianus , WANG Mingxuan , ZHOU Hualan , LI Zhenhai , WANG Jiguo , ZHANG Jianguo . Treatment of food wastewater using immobilized heterotrophic Chlorella vulgaris by Aspergillus niger pellets[J]. Food and Fermentation Industries, 2024 , 50(17) : 23 -30 . DOI: 10.13995/j.cnki.11-1802/ts.037408

References

[1] 李磊. 综合食品废水处理技术研究进展[J].皮革制作与环保科技, 2022(17):13-15.
LI L.Research progress of integrated food wastewater treatment technology[J].Leather Manufacture and Environmental Technology, 2022(17):13-15.
[2] 赵敏. 食品工业废水处理技术研究[J].现代食品, 2020(17):122-124.
ZHAO M.Study on wastewater treatment technology of food industry[J].Modern Food, 2020(17):122-124.
[3] 张智, 周健.食品工业高盐高氮磷有机废水处理技术发展趋势[J].给水排水, 2012, 38(9):1;51.
ZHANG Z, ZHOU J.Development trend of organic wastewater treatment technology with high salt, nitrogen and phosphorus in food industry[J].Water & Wastewater Engineering, 2012, 38(9):1;51.
[4] 邓永飞, 刘涛, 吴海铨, 等.食品工业废水处理技术研究进展[J].工业水处理, 2021, 41(10):1-7;13.
DENG Y F, LIU T, WU H Q, et al.Research progress of wastewater treatment technology in food industry[J].Industrial Water Treatment, 2021, 41(10):1-7;13.
[5] 陈柳州, 赵泉林, 叶正芳.食品工业废水处理技术研究进展[J].应用化工, 2022, 51(8):2332-2336.
CHEN L Z, ZHAO Q L, YE Z F.Research progress of wastewater treatment technology in food industry[J].Applied Chemical Industry, 2022, 51(8):2332-2336.
[6] ZHUANG L L, LI M T, HAO NGO H.Non-suspended microalgae cultivation for wastewater refinery and biomass production[J].Bioresource Technology, 2020, 308:123320.
[7] PHONG V H N, HAO N H, GUO W S, et al.Microalgae for saline wastewater treatment:A critical review[J].Critical Reviews in Environmental Science and Technology, 2020, 50(12):1224-1265.
[8] ROMERO VILLEGAS G I, FIAMENGO M, ACIÉN FERNÁNDEZ F G, et al.Outdoor production of microalgae biomass at pilot-scale in seawater using centrate as the nutrient source[J].Algal Research, 2017, 25:538-548.
[9] WREDE D, TAHA M, MIRANDA A F, et al.Co-cultivation of fungal and microalgal cells as an efficient system for harvesting microalgal cells, lipid production and wastewater treatment[J].PLoS One, 2014, 9(11):e113497.
[10] CHO H U, KIM Y M, PARK J M.Enhanced microalgal biomass and lipid production from a consortium of indigenous microalgae and bacteria present in municipal wastewater under gradually mixotrophic culture conditions[J].Bioresource Technology, 2017, 228:290-297.
[11] LIU H, LU Q, WANG Q, et al.Isolation of a bacterial strain, Acinetobacter sp.from centrate wastewater and study of its cooperation with algae in nutrients removal[J].Bioresource Technology, 2017, 235:59-69.
[12] NASIR N M, BAKAR N S, LANANAN F, et al.Treatment of African catfish, Clarias gariepinus wastewater utilizing phytoremediation of microalgae, Chlorella sp.with Aspergillus niger bio-harvesting[J].Bioresource Technology, 2015, 190:492-498.
[13] QIU Z D, YE Y X, YIN H, et al.An easily available biocarrier material mycelium pellet assembled with oms-2 for dye degradation[J].International Journal of Environmental Research, 2021, 15(1):93-103.
[14] EL-GENDI H, SALEH A K, BADIERAH R, et al.A comprehensive insight into fungal enzymes:Structure, classification, and their role in mankind’s challenges[J].Journal of Fungi, 2021, 8(1):23.
[15] ZHANG S, LI A, CUI D, et al.Performance of enhanced biological SBR process for aniline treatment by mycelial pellet as biomass carrier[J].Bioresource Technology, 2011, 102(6):4360-4365.
[16] MOHD HANAFIAH Z, WAN MOHTAR W H M, ABU HASAN H, et al.Performance of wild-Serbian Ganoderma lucidum mycelium in treating synthetic sewage loading using batch bioreactor[J].Scientific Reports, 2019, 9(1):16109.
[17] ZHANG B, WU L, GUO Y, et al.Rapid establishment of algal-bacterial granular sludge system by applying mycelial pellets in a lab-scale photo-reactor under low aeration conditions:Performance and mechanism analysis[J].Environmental Pollution, 2023, 322:121183.
[18] WANG Q R, HONG Y, LI L H.Insights into differences between spore-assisted and pellet-assisted microalgae harvesting using a highly efficient fungus:Efficiency, high-value substances, and mechanisms[J].The Science of the Total Environment, 2023, 877:162945.
[19] 郑潇, 周化岚, GULTOM Sarman Oktovianus, 等.黑曲霉快速高效收获异养小球藻的条件优化[J].食品与发酵工业, 2021, 47(23):16-23.
ZHENG X, ZHOU H L, OKTOVIANUS G, et al.Efficiently harvesting heterotrophic Chlorella vulgaris through co-culture and mixed mycelium of Aspergillus niger[J].Food and Fermentation Industries, 2021, 47(23):16-23.
[20] ZHANG J G, ZHANG J N.The filamentous fungal pellet and forces driving its formation[J].Critical Reviews in Biotechnology, 2016, 36(6):1066-1077.
[21] KURNIAWAN T A, LO W H, LIANG X, et al.Heavy metal removal from aqueous solutions using biomaterials and/or functional composites:Recent advances and the way forward in wastewater treatment using digitalization[J].Journal of Composites Science, 2023, 7(2):84.
[22] 吴思伟, 李思雨, 孙寒, 等.一株产胞外多糖微藻的分离鉴定及其多糖抗氧化活性的研究[J].食品与发酵工业, 2021, 47(24):193-200.
WU S W, LI S Y, SUN H, et al.Isolation and identification of an exopolysaccharide-producing microalgae strain and its antioxidant activity[J].Food and Fermentation Industries, 2021, 47(24):193-200.
[23] 孟丹阳, 杜艳, 陈复生.微藻中蛋白质的提取方法研究进展[J].食品与发酵工业, 2023, 49(21):346-357.
MENG D Y, DU Y, CHEN F S.Research progress on extraction methods of proteins from microalgae[J].Food and Fermentation Industries, 2023, 49(21):346-357.
[24] 孙建瑞, 姚琳琳, 李菁岚, 等.LED光质影响微藻生长和代谢产物积累的研究进展[J].食品与发酵工业, 2024, 50(2):321-328.
SUN J R, YAO L L, LI J L, et al.Effects of LED light quality on growth and metabolite accumulation in microalgae:A review[J].Food and Fermentation Industries, 2024, 50(2):321-328.
[25] NYYSSÖLÄ A, SUHONEN A, RITALA A, et al.The role of single cell protein in cellular agriculture[J].Current Opinion in Biotechnology, 2022, 75:102686.
[26] RITALA A, HÄKKINEN S T, TOIVARI M, et al.Single cell protein-state-of-the-art, industrial landscape and patents 2001-2016[J].Frontiers in Microbiology, 2017, 8:2009.
[27] KARPAGAM R, RAJ K J, ASHOKKUMAR B, et al.Characterization and fatty acid profiling in two fresh water microalgae for biodiesel production:Lipid enhancement methods and media optimization using response surface methodology[J].Bioresource Technology, 2015, 188:177-184.
[28] SONG Y L, WANG L J, QIANG X, et al.The promising way to treat wastewater by microalgae:Approaches, mechanisms, applications and challenges[J].Journal of Water Process Engineering, 2022, 49:103012.
[29] WAN Y J, YAN N, ZHAO J Q, et al.Trends and progress in Microalgae-based wastewater treatment technologies:A review[J].E3S Web of Conferences, 2021, 308:01014.
[30] LIU Y X, HU T T, ZHAO J, et al.Simultaneous removal of carbon and nitrogen by mycelial pellets of a heterotrophic nitrifying fungus-Penicillium sp.L1[J].Journal of Bioscience and Bioengineering, 2017, 123(2):223-229.
[31] WANG J J, TIAN Q H, ZENG W M, et al.Insights about fungus-microalgae symbiotic system in microalgae harvesting and wastewater treatment:A review[J].Renewable and Sustainable Energy Reviews, 2023, 182:113408.
[32] 李亚丽, 赵国强, 武双, 等.基于废水培养微藻及微藻资源化利用的研究[J].现代化工, 2021, 41(4):48-51.
LI Y L, ZHAO G Q, WU S, et al.Cultivation of microalage based on wastewater and utilization of microalage[J].Modern Chemical Industry, 2021, 41(4):48-51.
[33] CHAI W S, TAN W G, HALIMATUL MUNAWAROH H S, et al.Multifaceted roles of microalgae in the application of wastewater biotreatment:A review[J].Environmental Pollution, 2021, 269:116236.
[34] BROWN N, SHILTON A.Luxury uptake of phosphorus by microalgae in waste stabilisation ponds:Current understanding and future direction[J].Reviews in Environmental Science and Bio/Technology, 2014, 13(3):321-328.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.