Construction of engineered probiotic for degrading phenylalanine and analysis of its effect on feeding phenylketonuria mice

  • MA Xin ,
  • FU Jiafang ,
  • LIN Geng ,
  • ZHANG Peipei ,
  • CAO Guangxiang
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  • 1(Biomedical Sciences College & Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China)
    2(NHC Key Laboratory of biotechnology drugs (Shandong Academy of Medical Sciences), Jinan 250117, China)
    3(Key Lab for Rare & Uncommon Diseases of Shandong Province, Jinan 250117, China)

Received date: 2023-09-05

  Revised date: 2023-10-08

  Online published: 2024-10-10

Abstract

Phenylketonuria (PKU) is an autosomal recessive disease.A low-protein diet is the main treatment for PKU, and engineered probiotic is still in the stage of research and development.To explore the therapeutic effect of engineered probiotic on PKU, an engineered probiotic strain EcN-PAL expressing phenylalanine lyase was constructed based on Escherichia coli Nissle 1917 (EcN).The optimized codons of phenylalanine lyase gene was integrated into the genome of EcN by using the λ-Red homologous recombination system, and its function was verified by in vitro degradation experiment and mouse feeding experiment.Results showed that the phenylalanine lyase was successfully expressed in EcN-PAL, and the phenylalanine can be effectively degradated by EcN-PAL in vitro.The blood phenylalanine concentration in PKU mice showed a significant decrease when the mice was treated with EcN-PAL.The engineered probiotic E.coli EcN-PAL exhibited good ability to degrade phenylalanine, and could effectively treat PKU mice, laying a foundation for probiotic therapy in patients with PKU.

Cite this article

MA Xin , FU Jiafang , LIN Geng , ZHANG Peipei , CAO Guangxiang . Construction of engineered probiotic for degrading phenylalanine and analysis of its effect on feeding phenylketonuria mice[J]. Food and Fermentation Industries, 2024 , 50(17) : 75 -81 . DOI: 10.13995/j.cnki.11-1802/ts.037264

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