Naringenin is a natural flavonoid possessing good biological activities and therapeutic effects.However, naringenin has poor water solubility and low bioavailability, so it is very important to establish a simple and efficient delivery method for the application of naringenin.In this study, gallocatechin gallate (EGCG) covalently modified zein-hyaluronic acid (HA) composite nanoparticles loaded with naringenin were prepared by an anti-solvent precipitation method.Compared with gallic acid and tannic acid, the zein modified by EGCG had low free amino content and high polyphenol equivalent, and the modification effect was good.The size of the composite nanoparticles, which were formed via electrostatic interaction, hydrogen bonding and hydrophobic interaction, was between 180 and 240 nm, and the smallest particle size (183.62 nm) was obtained when the mass ratio of EGCG modified zein to HA was 10∶1.Due to the covalent modification of zein by EGCG, the encapsulation efficiency and loading capacity of the composite nanoparticles for naringenin were significantly improved, and the encapsulation efficiency was greater than 99% when the mass ratio of naringenin to the EGCG modified zein was 1∶10 or less.Structural characterization showed that most of naringenin was embedded inside the composite nanoparticles, and there were hydrogen bonds and hydrophobic interactions between naringenin, and EGCG or zein.The antioxidant activity of the composite nanoparticles loaded with naringenin was significantly higher than that of free naringenin, indicating that the EGCG modified zein-HA composite nanoparticles loaded with naringenin had an improved effect on the antioxidant activity of naringenin.
LI Wenjie
,
ZHAO Yuan
,
FU Lianfei
,
JIA Huiming
,
GUAN Ying
,
WANG Di
,
CHEN Chen
,
FAN Jinling
,
ZHANG Bin
. Fabrication and characterization of naringenin-loaded gallocatechin-gallate covalently modified zein-hyaluronic acid composite nanoparticles[J]. Food and Fermentation Industries, 2024
, 50(17)
: 139
-147
.
DOI: 10.13995/j.cnki.11-1802/ts.039097
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