Food and Fermentation Industries >

2024 , Vol. 50 >Issue 17: 186 - 197

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037326

Chemical composition, in vitro antioxidant, and skin-protective activities of Paeonia delavayi Franch stamen extract

  • LI Jin ,
  • DAI Manting ,
  • MOU Yaping ,
  • DU Chun ,
  • WANG Juan ,
  • WANG Zhenxing
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  • 1(College of landscape architecture and Horticulture, Southwest Forestry University, Kunming 650224, China)
    2(College of Forestry, Southwest Forestry University, Kunming 650224, China)
    3(Eco-Development Academy, Southwest Forestry University, Kunming 650224, China)
    4(College of Life Sciences, Southwest Forestry University, Kunming 650224, China)

Received date: 2023-09-10

  Revised date: 2023-10-16

  Online published: 2024-10-10

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Abstract

This study aimed to comprehensively analyze the chemical composition and biological activities of Paeonia delavayi Franch stamens by selecting red and yellow stamens at different developmental stages, including the transparent, initial blooming, and full blooming stages.Extraction was carried out using ethanol-water solutions with varying volume fractions (0%, 50%, 80%, and 100%).The total flavonoid and total phenolic contents of different Paeonia delavayi Franch stamen extracts were measured.The antioxidant activities were assessed in vitro by measuring the scavenging abilities of DPPH free radicals and ABTS cationic radicals, as well as ferric reducing power.The skin-protective activities were evaluated by measuring tyrosinase inhibition and elastase inhibition.Moreover, the chemical composition was analyzed via high-performance liquid chromatography (HPLC).Results demonstrated that the extraction rate, total phenolic and flavonoid contents, as well as the activities of antioxidant, tyrosinase inhibition, and elastase inhibition were highest in Paeonia delavayi Franch stamens when the ethanol volume fraction was 50%.In general, the chemical composition, in vitro antioxidant activities, and skin-protective activities of Paeonia delavayi Franch stamens exhibited dynamic accumulation and enhancement throughout the developmental stages, with the full blooming stage having the highest levels.Furthermore, the red stamens displayed superior chemical composition and biological activities compared to their yellow counterparts.Through HPLC, eleven compounds were identified.Of which, paeoniflorin, gallic acid, rutin, quercetin, and catechin were the primary phenolic compounds in Paeonia delavayi Franch stamens.This study offers a theoretical foundation for the development and application of Paeonia delavayi Franch stamens in various domains, including antioxidants, anti-wrinkle agents, and skin whitening treatments.

Key words： Paeonia delavayi Franch; stamen; chemical composition; antioxidant activity; tyrosinase inhibitory activity; elastase inhibitory activity

Cite this article

LI Jin , DAI Manting , MOU Yaping , DU Chun , WANG Juan , WANG Zhenxing . Chemical composition, in vitro antioxidant, and skin-protective activities of Paeonia delavayi Franch stamen extract[J]. Food and Fermentation Industries, 2024 , 50(17) : 186 -197 . DOI: 10.13995/j.cnki.11-1802/ts.037326

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