Food and Fermentation Industries >

2024 , Vol. 50 >Issue 18: 98 - 106

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039706

Screening of thermophilic Bacillus methylotrophicus for efficient 2,3-butanediol production and its fermentation optimization

  • LIN Yanhuan ,
  • CHEN Feixue ,
  • WEI Chenxin ,
  • LI Runzhi ,
  • ZHANG Liaoyuan ,
  • LIN Hui
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  • 1(College of Food Engineering, Zhangzhou Institute of Technology, Zhangzhou 363000, China)
    2(College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
    3(Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China)

Received date: 2024-04-26

  Revised date: 2024-06-05

  Online published: 2024-10-14

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Abstract

2,3-Butanediol, an important bulk chemical, is widely used in the field of chemical industry, food, pharmaceutical and fuel.Biological 2,3-butanediol production via microbial fermentation is the main method at present.However, several reported strains with high yields are opportunistic pathogen, and strict fermentation conditions are required, which limited the progress of biological 2,3-butanediol production.In this study, a newly isolated Bacillus methylotrophicus strain that thermophilically produces 2,3-butanediol was screened and identified from the soil of hot spring.The optimized fermentation conditions and medium components resulted in 2,3-butanediol production with 32.57 g/L at 24 h.Furthermore, the fermentation experiment in a 5 L bioreactor was performed, and the effects of different agitation speeds on 2,3-butanediol production were investigated.Ultimately, a two-stage fermentation strategy combined with fed-batch was developed, and 73.19 g/L 2,3-butanediol with the yield of 90.25% was achieved in a 5 L bioreactor at 45 h.

Key words： 2,3-butanediol; Bacillus methylotrophicus; thermophilic fermentation; fermentation optimization; fed-batch fermentation

Cite this article

LIN Yanhuan , CHEN Feixue , WEI Chenxin , LI Runzhi , ZHANG Liaoyuan , LIN Hui . Screening of thermophilic Bacillus methylotrophicus for efficient 2,3-butanediol production and its fermentation optimization[J]. Food and Fermentation Industries, 2024 , 50(18) : 98 -106 . DOI: 10.13995/j.cnki.11-1802/ts.039706

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