Food and Fermentation Industries >

2024 , Vol. 50 >Issue 18: 217 - 224

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037270

Antihypertensive mechanism of porcine collagen ACE inhibitory peptides based on network pharmacology and molecular docking

  • ZHOU Fating ,
  • LI Di ,
  • LI Kaifeng ,
  • JIANG Zhonggui ,
  • WEI Die ,
  • CONG Zhihui ,
  • CHEN Jingsheng ,
  • GU Xin ,
  • XIAO Guosheng
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  • (College of Biological and Food Engineering, Chongqing Three Gorges University, Chongqing 404100, China)

Received date: 2023-09-05

  Revised date: 2023-11-07

  Online published: 2024-10-14

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Abstract

This article investigated the mechanism of angiotensin converting enzyme (ACE) inhibitory peptides derived from porcine collagen in improving hypertension through network pharmacology and molecular docking.Bioinformatics tools were used to analyze and predict the properties of hypotensive peptides from porcine collagen sources.The potential targets of RL (Arg-Leu) and related targets of improving hypertension were screened by the database platform, and the potential targets of improving hypertension by active peptide RL were obtained by intersecting the two.The protein interaction network between common targets was constructed, and the common targets were annotated by the gene ontology (GO) function and enriched by the kyoto encyclopedia of genes and genomes (KEGG) pathway.Through molecular docking, the binding mechanism of the target peptide to the key target of hypertension was studied.Results showed that porcine collagen active peptide RL screened by the silicon method could play a variety of molecular functions in different cell components by regulating various biological processes.It played a role in lowering blood pressure through the PI3K-Akt signaling pathway, interleukin-17 (IL-17) signaling pathway, T cell receptor signaling pathway, MAPK signaling pathway, Wnt signaling pathway, and platelet activation pathway.It interacted with the key target ACE (6p0z) most statically and bound to the target protein through hydrogen bonding.It was mainly combined with various amino acid residues such as Asp-153, Gln-150, Glu-143, and so on, thus affecting the role of the target in each pathway, and was the best core target for the treatment of hypertension.

Key words： porcine collagen; bioactive peptide; high blood pressure; network pharmacology; molecular docking

Cite this article

ZHOU Fating , LI Di , LI Kaifeng , JIANG Zhonggui , WEI Die , CONG Zhihui , CHEN Jingsheng , GU Xin , XIAO Guosheng . Antihypertensive mechanism of porcine collagen ACE inhibitory peptides based on network pharmacology and molecular docking[J]. Food and Fermentation Industries, 2024 , 50(18) : 217 -224 . DOI: 10.13995/j.cnki.11-1802/ts.037270

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