Improving effect of peach gum polysaccharide on constipation model mice

  • GAO Fan ,
  • SU Jie ,
  • ZHOU Hengpu ,
  • LI Jin ,
  • YING Guowei ,
  • CHEN Suhong ,
  • LYU Guiyuan
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  • 1(School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China)
    2(College of Pharmaceutical Science,Zhejiang University of Technology, Hangzhou 310014, China)

Received date: 2024-04-25

  Revised date: 2024-07-03

  Online published: 2024-10-29

Abstract

To investigate the effect and mechanism of Peach gum polysaccharide (PGP) in relieving constipation.Constipation model mice were established by continuous intragastric administration of loperamide hydrochloride (10 mg/kg BW) for 14 days, and peach gum polysaccharides (100 and 300 mg/kg) were given for 3 weeks after the model was successful.The number of fecal grains, fecal water content, first black stool time, intestinal propulsion rate, gastric emptying rate and behavioral indexes were measured within 6 hours during administration.The contents of Gastrin (GAS), Motilin (MTL) and 5-hydroxytryptamine (5-HT) in serum were determined by ELISA.The number of goblet cells in colon tissues was observed by Alcian blue-periodate Scheff (AB-PAS) staining.The colonic morphology was observed by H&E staining.The expression of 5-HT3R (5-HT3R) in colon tissue of mice was observed by immunohistochemistry.The expression of tight junction proteins Occludin and Claudin-1 in colon tissue of mice was observed by immunofluorescence method.The relative expression of Occludin, 5-HT3R and tryptophan hydroxylase 1 (TPH-1) in colon tissue was determined by Western blot.Peach gum can significantly increase the grasping power, the number of autonomous activities, the number of open arm entry and the stay time of open arm in mice with constipation model.Increase fecal grain count, fecal moisture content and gastric emptying rate, shorten the time of first black stool;Increase the levels of GAS and MTL in serum;Increase the number of goblet cells in colon tissue, improve intestinal mucosal injury, and up-regulate the expression of Occludin and Claudin-1 proteins;The level of 5-HT in serum and the expression of 5-HT3R and TPH-1 protein in colon tissue were increased.Peach gum polysaccharide can ameliorate constipation, and its mechanism may be related to promoting gastrointestinal hormone secretion and activating 5-HT signaling pathway.

Cite this article

GAO Fan , SU Jie , ZHOU Hengpu , LI Jin , YING Guowei , CHEN Suhong , LYU Guiyuan . Improving effect of peach gum polysaccharide on constipation model mice[J]. Food and Fermentation Industries, 2024 , 50(19) : 40 -48 . DOI: 10.13995/j.cnki.11-1802/ts.039687

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