Food and Fermentation Industries >

2024 , Vol. 50 >Issue 20: 303 - 312

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039887

Chemical composition analysis and multi-component content determination of grape seed extract based on ultra performance liquid chromatography tandem mass spectrometry

  • LI Jinfa ,
  • DIAO Juanjuan ,
  • CHEN Zhanghao ,
  • GAO Shuang ,
  • CHANG Junmin
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  • 1(School of Pharmacy, Xinjiang Medical University, Urumqi 830011, China)
    2(Central Laboratory, Xinjiang Medical University, Urumqi 830011,China)
    3(Xinjiang Key Laboratory of Natural Drug Active Components and Drug Release Technology, Urumqi 830011, China)

Received date: 2024-05-15

  Revised date: 2024-07-15

  Online published: 2024-11-01

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Abstract

In this study, liquid chromatography-mass spectrometry (LC-MS) combined with the UNIFI screening platform was used to quickly and qualitatively analyze the chemical components in grape seed extract, and establish a method for the determination of effective components in grape seed extract.Firstly, the mass spectrum data of grape seed extract were collected by ultra-performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry (UPLC-Q-TOF-MS/MS).According to the matching results of the database built by UNIFI software, combined with the retention time of control substance, characteristic fragment ions and related literature, 20 chemical constituents were identified from grape seed extract, including 7 polyphenols, 1 triterpene, 1 flavonol, 1 unsaturated fatty acid, 1 polyhydroxylamine, 3 phenolic acids, and 6 amino acids.In addition, the four active components (catechin, epicatechin, proanthocyanidin B2 and proanthocyanidin C1) in grape seed extract were determined simultaneously by ultra performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS).The linear relationship was good in their respective ranges (r≥0.998), and the average sample recovery was 96.7%-102.6%.This study provides a rapid, simple, and accurate method for qualitative and quantitative analysis of chemical components in grape seed extract, providing an important theoretical reference for its quality evaluation and basic research of pharmacodynamic substances.

Key words： grape seed extract; chemical composition analysis; content determination; ultra performance liquid chromatography tandem mass spectrometry

Cite this article

LI Jinfa , DIAO Juanjuan , CHEN Zhanghao , GAO Shuang , CHANG Junmin . Chemical composition analysis and multi-component content determination of grape seed extract based on ultra performance liquid chromatography tandem mass spectrometry[J]. Food and Fermentation Industries, 2024 , 50(20) : 303 -312 . DOI: 10.13995/j.cnki.11-1802/ts.039887

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