In order to further enhance the production yield of phytase by Pichia pastoris and improve the utilization efficiency of raw materials, the online biomass monitoring technology was introduced to the study of the fermentation process.After validating the excellent linear correlation between capacitance values and viable cell concentration, this study utilized capacitance values and conductivity values reflected by the online biomass sensor, and the physiological parameter oxygen uptake rate, to investigate the impact of nutrient salt content and methanol feeding rates during the induction phase on phytase production.The results indicated that under the optimized conditions of 75% nutrient salt content and a maximum methanol feeding rate of 1∶10(duty cycle), the production titer and total activity of phytase at the end of fermentation reached 16 900 U/mL and 853.39×106 U, respectively, representing 29.8% and 42.6% increases compared to the unoptimized conditions.
XU Hongfei
,
LI Lianwei
,
WANG Fengchao
,
LI Chao
,
LI Yuqiang
,
WANG Yonghong
. Optimization of fermentation process for producing phytase by Pichia pastoris with an online biomass sensor[J]. Food and Fermentation Industries, 2024
, 50(21)
: 45
-50
.
DOI: 10.13995/j.cnki.11-1802/ts.038147
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