The application of bitter peptides in the food industry is limited due to their strong bitterness.In this study, bitterness masking bitter peptide double emulsion microcapsules were prepared by spray drying technology through double emulsion microcapsule system.The applicability of microcapsules in food application and production was analyzed by evaluating the physical and chemical properties and bitterness masking effect of microcapsules.The results of scanning electron microscopy showed that the bitter peptide double emulsion microcapsules had a uniform and complete spherical structure.The specific surface area of the microcapsule powder was (0.155±0.002 3) m2/g, the pore volume was (0.002±0.000 6) cm3/g, and the average pore size was (21.121±0.001 2) nm.The moisture content, solubility, surface oil content results showed that the system had a good encapsulation effect of bitter peptide, and the loading amount of bitter peptide was 10%.The result of microstructure and stability of microcapsule reconstituted emulsion showed that the preparation of bitter peptide double emulsion microcapsules by spray drying technology had good application feasibility.The consequences of the sensory evaluation showed that the system could significantly reduce consumers' perception of bitterness in bitter peptides and achieve the purpose of masking bitterness.This research provides a theoretical foundation for the development and application of bitter masking peptides.
[1] AKBARBAGLU Z, TAMJIDI F, SARABANDI K, et al.Physicochemical characteristics and antioxidant stability of spray-dried soy peptide fractions[J].Food Science & Nutrition, 2023, 11(7):3949-3958.
[2] ALIM A, SONG H L, YANG C, et al.Changes in the perception of bitter constituents in thermally treated yeast extract[J].Journal of the Science of Food and Agriculture, 2019, 99(10):4651-4658.
[3] BERTELSEN A S, LAURSEN A, KNUDSEN T A, et al.Bitter taste masking of enzyme-treated soy protein in water and bread[J].Journal of the Science of Food and Agriculture, 2018, 98(10):3860-3869.
[4] 贾聪, 孟醒, 游静, 等.低苦味芝麻ACE抑制肽的制备及氨基酸组成和结构分析[J].食品与发酵工业, 2021, 47(17):172-178.
JIA C, MENG X, YOU J, et al.Preparation, amino acid composition and structure analysis of low bitterness sesame ACE inhibitory peptides[J].Food and Fermentation Industries, 2021, 47(17):172-178.
[5] LIU J B, LIU M Y, HE C C, et al.Effect of thermal treatment on the flavor generation from Maillard reaction of xylose and chicken peptide[J].LWT - Food Science and Technology, 2015, 64(1):316-325.
[6] MIRZAPOUR-KOUHDASHT A, MCCLEMENTS D J, TAGHIZADEH M S, et al.Strategies for oral delivery of bioactive peptides with focus on debittering and masking[J].NPJ Science of Food, 2023, 7(1):22.
[7] ENSIGN L M, CONE R, HANES J.Oral drug delivery with polymeric nanoparticles:The gastrointestinal mucus barriers[J].Advanced Drug Delivery Reviews, 2012, 64(6):557-570.
[8] MCCLEMENTS D J.Encapsulation, protection, and release of hydrophilic active components:Potential and limitations of colloidal delivery systems[J].Advances in Colloid and Interface Science, 2015, 219:27-53.
[9] XIA T H, XUE C H, WEI Z H.Physicochemical characteristics, applications and research trends of edible Pickering emulsions[J].Trends in Food Science & Technology, 2021, 107:1-15.
[10] 龙海涛, 毕阳, 张慧秀, 等.冷冻干燥制备番茄红素微胶囊及其缓释性能研究[J].食品与发酵工业, 2016, 42(12):125-131.
LONG H T, BI Y, ZHANG H X, et al.Microcapsulation of lycopene by freeze-drying method and its slow-release performance[J].Food and Fermentation Industries, 2016, 42(12):125-131.
[11] CHOI S J, DECKER E A, MCCLEMENTS D J.Impact of iron encapsulation within the interior aqueous phase of water-in-oil-in-water emulsions on lipid oxidation[J].Food Chemistry, 2009, 116(1):271-276.
[12] 蒲晓璐. w/o/w双乳结构调控及在益生菌递送中的应用进展[J].食品与发酵工业, 2023, 49(11):280-287.
PU X L.A review in the structure manipulation of w/o/w double emulsions and applications in probiotics delivery[J].Food and Fermentation Industries, 2023, 49(11):280-287.
[13] DESAI K G H, JIN PARK H.Recent developments in microencapsulation of food ingredients[J].Drying Technology, 2005, 23(7):1361-1394.
[14] 胡丽萍, 李辉玉, 林嘉欣, 等.基于喷雾干燥法益生菌-槲皮素的共微囊化及其耐消化性研究 [J/OL].食品与发酵工业, 2023.DOI:10.13995/j.cnki.11-1802/ts.036942.
HU L P, LI H Y, LIN J X, et al.Co-microencapsulation of probiotic-quercetin based on spray-drying and its digestibility resistance study[J/OL].Food and Fermentation Industries, 2023.DOI:10.13995/j.cnki.11-1802/ts.036942.
[15] ZHU Y Q, CHEN X, MCCLEMENTS D J, et al.pH-, ion- and temperature-dependent emulsion gels:Fabricated by addition of whey protein to gliadin-nanoparticle coated lipid droplets[J].Food Hydrocolloids, 2018, 77:870-878.
[16] LI X, WANG X, XU D X, et al.Influence of calcium-induced droplet heteroaggregation on the physicochemical properties of oppositely charged lactoferrin coated lutein droplets and whey protein isolate-coated DHA droplets[J].Food & Function, 2017, 8(8):2748-2759.
[17] MELGOSA R, BENITO-ROMÁN Ó, SANZ M T, et al.Omega-3 encapsulation by PGSS-drying and conventional drying methods.Particle characterization and oxidative stability[J].Food Chemistry, 2019, 270:138-148.
[18] SHEU T Y, ROSENBERG M.Microstructure of microcapsules consisting of whey proteins and carbohydrates[J].Journal of Food Science, 1998, 63(3):491-494.
[19] LOCALI PEREIRA A R, GONÇALVES CATTELAN M, NICOLETTI V R.Microencapsulation of pink pepper essential oil:Properties of spray-dried pectin/SPI double-layer versus SPI single-layer stabilized emulsions[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects, 2019, 581:123806.
[20] GAO Y, LI X Q, XIE Y F, et al.Encapsulation of bitter peptides in diphasic gel double emulsions:Bitterness masking, sustained release and digestion stability[J].Food Research International, 2022, 162:112205.
[21] URSICA L, TITA D, PALICI I, et al.Particle size analysis of some water/oil/water multiple emulsions[J].Journal of Pharmaceutical and Biomedical Analysis, 2005, 37(5):931-936.
[22] DAMMAK I, DO AMARAL SOBRAL P J.Effect of different biopolymers on the stability of hesperidin-encapsulating O/W emulsions[J].Journal of Food Engineering, 2018, 237:33-43.
[23] TAN C, WANG J, SUN B G.Biopolymer-liposome hybrid systems for controlled delivery of bioactive compounds:Recent advances[J].Biotechnology Advances, 2021, 48:107727.
[24] LU H, WANG J, HUANG M G, et al.Bitterness-masking assessment of luteolin encapsulated in whey protein isolate-coated liposomes[J].Food & Function, 2023, 14(7):3230-3241.
