Food and Fermentation Industries >

2024 , Vol. 50 >Issue 21: 333 - 340

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040495

Determination of 94 kinds of pesticide residues in eggs by ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry

  • XIAO Yong ,
  • ZENG Xiaoming ,
  • LI Zheng ,
  • YUAN Liejiang ,
  • DENG Hang ,
  • WANG Shuxia ,
  • PAN Zhao
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  • (Hunan Province Produce Commodity Quality Testing & Research Institute, Changsha 410007,China)

Received date: 2024-07-16

  Revised date: 2024-07-29

  Online published: 2024-11-28

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Abstract

A method for determination of 94 kinds of pesticide residues in eggs by ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q/Orbitrap HRMS)was established.The egg samples were extracted with 1% acetate acetonitrile and purified with anhydrous magnesium sulfate and C18 adsorbents.The analytes were separated on a Accucore aQ column and the positive and negative ions were scanned in the Full MS-ddMS2 mode.Quantification was performed by the chromatographic peak area of the extracted primary parent ions, and qualitative was performed by the retention time and secondary fragment ion obtained by data-dependent product ion scanning.The 94 kinds of pesticides showed good linearities within their respective line arranges, with a correlation coefficient greater than 0.995.The limit of quantification (LOQ) of the method were 1.0-10.0 μg/kg.The recoveries at three different spiking levels were 68% to 108%, with relative standard deviations of 2.6% to 14.6%.The method is a simple, accurate and reliable, and suitable for quantitative screening of 94 kinds of pesticide residues in eggs.

Key words： ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry; egg; pesticide residue

Cite this article

XIAO Yong , ZENG Xiaoming , LI Zheng , YUAN Liejiang , DENG Hang , WANG Shuxia , PAN Zhao . Determination of 94 kinds of pesticide residues in eggs by ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry[J]. Food and Fermentation Industries, 2024 , 50(21) : 333 -340 . DOI: 10.13995/j.cnki.11-1802/ts.040495

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