Application of loop-mediated isothermal amplification in the detection of food safety

  • AN Hongyu ,
  • TAO Ze ,
  • CHEN Chen ,
  • LU Zenghui ,
  • WAN Shiyuan ,
  • LI Tuoping ,
  • LI Suhong
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  • 1(College of Food Science, Shenyang Agricultural University, Shenyang 110886, China)
    2(Central Grain Reserve Xinmin Direct Warehouse Co.Ltd., Xinmin 110300, China)
    3(State Grain Reserves Xuxinzhuang(Beijing)Depot Co.Ltd., Beijing 101119, China)

Received date: 2023-11-08

  Revised date: 2023-12-12

  Online published: 2024-11-28

Abstract

Food safety is a major and enduring challenge with far-reaching implications for the quality of human life.Loop-mediated isothermal amplification is an efficient, sensitive, fast and highly specific technique for nucleic acid isothermal amplification.Compared with traditional nucleic acid amplification methods, it designs 4 different primers for 6 regions on the target DNA strand.Sample DNA, primers, Bst DNA polymerase, dNTPs, Mg2+, etc.are added to the reaction tube.Nucleic acid amplification is performed at 60-65 ℃ for 30-60 min and can be accomplished quickly in a single step.It is widely used in food safety testing to detect the presence of foodborne pathogenic bacteria contamination, food adulteration, genetically modified food, and food allergens.In this paper, the principle of LAMP technology, the comparison of the main features with PCR technology, the existing problems and the innovative applications in the field of food safety detection are reviewed to provide a reference for its application and promotion in the future.

Cite this article

AN Hongyu , TAO Ze , CHEN Chen , LU Zenghui , WAN Shiyuan , LI Tuoping , LI Suhong . Application of loop-mediated isothermal amplification in the detection of food safety[J]. Food and Fermentation Industries, 2024 , 50(21) : 388 -396 . DOI: 10.13995/j.cnki.11-1802/ts.037897

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