Food and Fermentation Industries >

2024 , Vol. 50 >Issue 22: 28 - 36

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037951

Ultrasonic-alkaline extraction, characterization, and antioxidant activity of paramylon from Euglena gracilis

  • HU Fengyang ,
  • LI Shichang ,
  • SONG Gendi ,
  • DING Hui ,
  • CHANG Yingjie ,
  • GONG Minggui
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China)
    2(Henan Provincial Food Microbiology Engineering Technology Research Center, Luoyang 471023, China)
    3(Department of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang 471023, China)
    4(Luoyang Ouke Baike Biotechnology Co.Ltd., Luoyang 471023, China)

Received date: 2023-11-14

  Revised date: 2024-01-16

  Online published: 2024-12-17

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Abstract

Euglena gracilis polysaccharide (EGP), as a new natural functional food, has attracted more and more attention.This study investigated the extraction yield, structural characterization, and in vitro antioxidant activity of EGP.The extraction yield EGP was optimized with ultrasonic-alkali extraction by Plackett-Burman and response surface design.The molecular weight, monosaccharide composition, group composition, microstructure, and antioxidant activity of EGP were analyzed using gel permeation chromatography (GPC), ion chromatography (IC), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), and free radical scavenging tests.The optimum process conditions were as follows:the liquid-to-material ratio of 1∶40 (g∶mL), water bath temperature of 41 ℃, water bath time of 0.9 h, NaOH solution concentration of 1 mol/L, ultrasonic power of 120 W, and ultrasonic time of 3 min.Under these conditions, the extraction yield was (62.84±0.49)%.Results indicated that EGP was composed of 100% glucose and connected by β-glycosidic bonds, and the molecular weight were 2.13×104 Da.Antioxidant experiments demonstrated that EGP showed good antioxidant activity against ·OH, DPPH free radicals, ABTS cationic radicals, and ·O-2.The structural study of EGP provides a scientific theoretical basis for the continued development and utilization of Euglena gracilis.

Key words： Euglena gracilis; Euglena gracilis polysaccharide; response surface method; structural characterization; antioxidant activity

Cite this article

HU Fengyang , LI Shichang , SONG Gendi , DING Hui , CHANG Yingjie , GONG Minggui . Ultrasonic-alkaline extraction, characterization, and antioxidant activity of paramylon from Euglena gracilis[J]. Food and Fermentation Industries, 2024 , 50(22) : 28 -36 . DOI: 10.13995/j.cnki.11-1802/ts.037951

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