Proteases are considered as clean alternatives to replace chemicals to use in the leather dehairing process to avoid serious pollution. This study aimed to optimize the condition for protease production by Bacillus amyloliquefaciens BS5582 strain, to characterize the enzymatic properties and to evaluate its application in cattle hide dehairing.Results showed that the optimal enzyme production media by BS5582 strain were corn powder 50.0 g/L, soybean powder 40.0 g/L, Na2HPO4·12H2O 6.0 g/L, KH2PO4 3.0 g/L, MgSO4 1.8 g/L, and CaCl2 0.75 g/L, with the initial pH 6.The optimal fermentation condition was as follows:The fermentation temperature was 35 ℃, the inoculation volume was 10%, and the loading volume was 15 mL.Under this condition, the crude enzyme activity of the enzyme solution reached (5 270.59±19.61) U/mL, which was 21 folds higher than that before optimization.The optimum temperature and pH of the proteases produced by the BS5582 strain were 40 ℃ and 8.5, respectively.Mn2+ had a significant activation effect on protease activity while Fe2+, Fe3+, and PMSF significantly inhibited the enzyme activity.The protease showed good cattle skin hair removal ability with little collagen damage.Compared with traditional chemical dehairing, the application of proteases had no hair root remaining in the pores of cattle skin with the falling of intact hairs.The pollution indexes of the dehairing wastewater, such as total suspended solids, chemical oxygen demand, and five-day biochemical oxygen demand, were reduced by 87%, 39%, and 45%, respectively.This indicated that the proteases produced by B.amyloliquefaciens BS5582 were of great potential for application in the leather industry.
REN Tao
,
NIU Chengtuo
,
ZHENG Feiyun
,
LIU Chunfeng
,
WANG Jinjing
,
LI Qi
. Fermentation optimization and enzymatic property analysis of proteases produced by Bacillus amyloliquefaciens BS5582 and its application in cattle skin dehairing[J]. Food and Fermentation Industries, 2024
, 50(22)
: 106
-114
.
DOI: 10.13995/j.cnki.11-1802/ts.038247
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