Food and Fermentation Industries >

2024 , Vol. 50 >Issue 22: 159 - 166

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037954

Production of bioethanol from polysaccharides in Grateloupia livida based on dilute acid pretreatment and Pichia kudriavzevii fermentation

  • QIU Yi ,
  • YANG Xianqing ,
  • LI Chunsheng ,
  • QI Bo ,
  • ZHAO Yongqiang ,
  • PAN Chuang ,
  • CHEN Shengjun
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China)
    2(Key Laboratory of Aquatic Products Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China)

Received date: 2023-11-15

  Revised date: 2024-01-15

  Online published: 2024-12-17

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Abstract

Fermentation of Grateloupia livida has the potential to produce bioethanol because it is rich in polysaccharides and low price.In this study, G.livida was used as raw material for bioethanol production.The optimal pretreatment condition for hydrolysis of G.livida by dilute sulfuric acid was obtained through the single factor and response surface test, including the solid-liquid ratio of 1∶20 (g∶mL), hydrolysis time of 1.8 h, hydrolysis temperature of 100 ℃, and sulfuric acid mass fraction of 3%, in which condition the reducing sugar content reached the maximum (37.64%).The compositions and contents of monosaccharides in the hydrolysate of G.livida pretreated with dilute sulfuric acid were analyzed.Results showed that dilute sulfuric acid pretreatment could significantly increase the content of monosaccharides, among which galactose and glucose possessed the highest contents.The hydrolysate of G.livida was further fermented by Pichia kudriavzevii.This yeast exhibited good growth and ethanol production ability, and the increase of inoculation could significantly enhance its biomass and ethanol fermentation characteristics.Pearson correlation analysis showed that the contents of monosaccharides in the hydrolysate of G.livida were significantly negatively correlated with biomass and various ethanol fermentation parameters, while the biomass was significantly positively correlated with various ethanol fermentation parameters.It indicated that the consumption of monosaccharides played an important role in the growth and ethanol production of P.kudriavzevii, and the growth of yeast was directly related to the production of ethanol fermentation.The correlation network showed that the consumption of glucose and galactose had the greatest influence on the biomass increase and ethanol production of P.kudriavzevii.These results suggest that the use of G.livida as raw material through dilute acid pretreatment combined with P.kudriavzevii fermentation has an application potential to produce bioethanol, which also provides an important technical reference for the fermentation of other algae to produce bioethanol.

Key words： Grateloupia livida; acid hydrolysis; bioethanol; Pichia kudriavzevii; reducing sugar content; monosaccharide composition; correlation network

Cite this article

QIU Yi , YANG Xianqing , LI Chunsheng , QI Bo , ZHAO Yongqiang , PAN Chuang , CHEN Shengjun . Production of bioethanol from polysaccharides in Grateloupia livida based on dilute acid pretreatment and Pichia kudriavzevii fermentation[J]. Food and Fermentation Industries, 2024 , 50(22) : 159 -166 . DOI: 10.13995/j.cnki.11-1802/ts.037954

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