Food and Fermentation Industries >

2024 , Vol. 50 >Issue 22: 197 - 203

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038041

Metabolomics-based analysis of Lentilactobacillus kefiri IMAU10804 metabolite differences before and after acid stress

  • WANG Huiying ,
  • BO Xiaoyu ,
  • YUAN Baoyi ,
  • BAO Qiuhua
Expand
  • (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot 010018, China)

Received date: 2023-11-22

  Revised date: 2024-01-10

  Online published: 2024-12-17

Fold

Abstract

Acid stress restricts the viability and probiotic functions of lactic acid bacteria during production applications.Therefore, studying the potential acid tolerance mechanisms of a specific strain is crucial for its further application.This study first examined the influence of decreasing acidity on the growth of Lentilactobacillus kefiri IMAU10804, a slow-growing milk rod bacterium.Subsequently, this study used liquid chromatography-mass spectrometry (LC-MS) to analyze the metabolomics differences in L.kefiri IMAU10804 before and after acid stress.Results showed that the growth of L.kefiri IMAU10804 was inhibited with decreasing pH.When L.kefiri IMAU10804 was treated under pH 3.0 for 60 min, 796 and 615 metabolites were detected in positive and negative ion modes, respectively.Multivariate statistical analysis was performed using SIMCA 14.1 software combined with Metaboanalyst 5.0, revealing that a large number of metabolites were enriched in metabolic pathways, such as ABC transporter, arginine and proline metabolism, and purine metabolism.Based on the criteria of P<0.05, variable importance in projection score ≥ 1, and fold change ≥ 1.5, 24 major differential metabolites were found, including L-proline, aspartic acid, and trehalose.This study demonstrated significant changes in the growth and metabolism of L.kefiri IMAU10804 under acid stress environment, providing data support for the subsequent development and application of lactic acid bacteria products.

Key words： Lentilactobacillus; Lentilactobacillus kefiri IMAU10804; acid stress; metabolomics; acid tolerance mechanisms

Cite this article

WANG Huiying , BO Xiaoyu , YUAN Baoyi , BAO Qiuhua . Metabolomics-based analysis of Lentilactobacillus kefiri IMAU10804 metabolite differences before and after acid stress[J]. Food and Fermentation Industries, 2024 , 50(22) : 197 -203 . DOI: 10.13995/j.cnki.11-1802/ts.038041

References

[1] WANG Y Q, WU J T, LV M X, et al. Metabolism characteristics of lactic acid bacteria and the expanding applications in food industry[J]. Frontiers in Bioengineering and Biotechnology, 2021, 9: 612285.
[2] 何进, 徐思杨, 刘波, 等. 乳酸菌在农业和食品加工中的应用研究进展[J]. 微生物学杂志, 2022, 42(4): 1-11.
HE J, XU S Y, LIU B, et al. Advance on applied researches of lactic acid bacteria in agriculture and food industry[J]. Journal of Microbiology, 2022, 42(4): 1-11.
[3] GUAN N Z, LIU L. Microbial response to acid stress: mechanisms and applications[J]. Applied Microbiology and Biotechnology, 2020, 104(1): 51-65.
[4] WU C D, HUANG J, ZHOU R Q. Progress in engineering acid stress resistance of lactic acid bacteria[J]. Applied Microbiology and Biotechnology, 2014, 98(3): 1055-1063.
[5] WANG H Y, ZHANG Y H, DAI L X, et al. Metabolomic differences between viable but nonculturable and recovered Lacticaseibacillus paracasei Zhang[J]. Foods, 2023, 12(18): 3472.
[6] 李娜. L. rhamnosus太空诱变株R7970-3发酵牛乳的研制及代谢组学研究[D]. 呼和浩特: 内蒙古农业大学, 2023.
LI N. Development of milk fermented by L. rhamnosus space mutant strain R7970-3 and metabolomics study[D]. Hohhot: Inner Mongolia Agricultural University, 2023.
[7] 郑莎莎. 干酪乳杆菌H1发酵红酸汤的品质影响及特征代谢物研究[D]. 贵阳: 贵州大学, 2020.
ZHENG S S. Study on quality influence and characteristic metabolites of tomato sour soup fermented by Lactobacillus casei H1[D]. Guiyang: Guizhou University, 2020.
[8] 张筠, 孟祥晨. 乳酸菌的胁迫应答及其对碳水化合物代谢的影响[J]. 中国食品学报, 2017, 17(6): 145-151.
ZHANG J, MENG X C. Stress responses and impact of carbonhydrate metabolism in lactic acid bacteria[J]. Journal of Chinese Institute of Food Science and Technology, 2017, 17(6): 145-151.
[9] ZHENG J S, WITTOUCK S, SALVETTI E, et al. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae[J]. International Journal of Systematic and Evolutionary Microbiology, 2020, 70(4): 2782-2858.
[10] 张栋, 赵彤, 荀一萍, 等. 一株开菲尔乳杆菌的分离及特性研究[J]. 食品科技, 2018, 43(8): 15-18.
ZHANG D, ZHAO T, XUN Y P, et al. Isolation and properties of Lactococcuslactis kefiri[J]. Food Science and Technology, 2018, 43(8): 15-18.
[11] CARASI P, MALAMUD M, SERRADELL M A. Potentiality of food-isolated Lentilactobacillus kefiri strains as probiotics: State-of-art and perspectives[J]. Current Microbiology, 2021, 79(1):21.
[12] MANNA L, RIZZI E, BAFILE E, et al. Lentilactobacillus kefiri SGL 13 and andrographis paniculata alleviate dextran sulfate sodium induced colitis in mice[J]. Frontiers in Nutrition, 2023, 10: 1072334.
[13] 包秋华, 王丽娜, 张雨虹, 等. 益生菌干酪乳杆菌Zhang VBNC态和正常态的代谢组学研究[J]. 中国乳品工业, 2022, 50(6): 10-15.
BAO Q H, WANG L N, ZHANG Y H, et al. Metabonomics study of probiotic Lactobacillus casei Zhang VBNC state and normal state[J]. China Dairy Industry, 2022, 50(6): 10-15.
[14] 王道滇, 肖梦林, 魏光强, 等. 基于干酪乳杆菌ZLC01的山羊奶发酵乳及其特征性代谢物研究[J]. 食品与发酵工业, 2024, 50(9):71-80.
WANG D D, XIAO M L, WEI G Q, et al. Fermented goat milk with Lactobacillus casei ZLC01 and its characteristic metabolites[J]. Food and Fermentation Industries, 2024, 50(9):71-80.
[15] 林亚男. 亚压力胁迫对Lactobacillus acidophilus LA-5营养消耗模式的影响[D]. 哈尔滨: 东北农业大学, 2022.
LIN Y N. The effect of sub-stress on the nutrient consumption pattern of Lactobacillus acidophilus LA-5[D]. Harbin: Northeast Agricultural University, 2022.
[16] SUN Y R, PENG C T, WANG J C, et al. Metabolic footprint analysis of volatile metabolites to discriminate between different key time points in the fermentation and storage of starter cultures and probiotic Lactobacillus casei Zhang milk[J]. Journal of Dairy Science, 2021, 104(3): 2553-2563.
[17] 马立清, 张文羿, 孙天松. 嗜热链球菌S4发酵乳代谢组学的研究[J]. 食品与发酵工业, 2021, 47(19): 71-78.
MA L Q, ZHANG W Y, SUN T S. Metabolomics analysis of fermented milk with Streptococcus thermophilus S4[J]. Food and Fermentation Industries, 2021, 47(19): 71-78.
[18] 孙雷. 鼠李糖乳酪杆菌太空诱变株R7970-3在发酵羊乳中的应用研究[D]. 呼和浩特: 内蒙古农业大学, 2023.
SUN L. Study on the application of Lacticaseibacillus rhamnosus space mutant strain R7970-3 in fermented goat milk[D]. Hohhot: Inner Mongolia Agricultural University, 2023.
[19] ZHU Z M, YANG J H, YANG P S, et al. Enhanced acid-stress tolerance in Lactococcus lactis NZ9000 by overexpression of ABC transporters[J]. Microbial Cell Factories, 2019, 18(1): 136.
[20] WANG W W, HE J Y, PAN D D, et al. Metabolomics analysis of Lactobacillus plantarum ATCC 14917 adhesion activity under initial acid and alkali stress[J]. PLoS One, 2018, 13(5): e0196231.
[21] MIYAJIMA, M. Amino acids: Key sources for immunometabolites and immunotransmitters[J]. International Immunology, 2020, 32(7): 435-446.
[22] QIAO Y L, LIU G F, LENG C, et al. Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations[J]. Scientific Reports, 2018, 8(1): 12441.
[23] LI H, MA M L, LUO S, et al. Metabolic responses to ethanol in Saccha-romyces cerevisiae using a gas chromatography tandem mass spectrome-try-based metabolomics approach[J]. The International Journal of Biochemistry & Cell Biology, 2012, 44(7): 1087-1096.
[24] ZHANG J, CAIYIN Q, FENG W J, et al. Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44[J]. Scientific Reports, 2016, 6: 27973.
[25] 杨友馨, 王瑞雪, 王俊国. 海藻糖对乳酸菌保护机制的研究进展[J]. 乳业科学与技术, 2021, 44(6): 45-50.
YANG Y X, WANG R X, WANG J G. Progress in research on the protective mechanism of trehalose on lactic acid bacteria[J]. Journal of Dairy Science and Technology, 2021, 44(6): 45-50.
[26] SUN Z K, YUE Z H, LIU E, et al. Assessment of the bifidogenic and antibacterial activities of xylooligosaccharide[J]. Frontiers in Nutrition, 2022, 9: 858949.
[27] WU C D, ZHANG J, WANG M, et al. Lactobacillus casei combats acid stress by maintaining cell membrane functionality[J]. Journal of Industrial Microbiology and Biotechnology, 2012, 39(7): 1031-1039.
[28] TANG Z W, YE W R, CHEN H T, et al. Role of purines in regulation of metabolic reprogramming[J]. Purinergic Signalling, 2019, 15(4): 423-438.
[29] MAAN K, BAGHEL R, DHARIWAL S, et al. Metabolomics and transcriptomics based multi-omics integration reveals radiation-induced altered pathway networking and underlying mechanism[J]. NPJ Systems Biology and Applications, 2023, 9(1): 42.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.