Food and Fermentation Industries >

2024 , Vol. 50 >Issue 22: 278 - 285

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038205

Optimization of mycelium liquid-state cultivation conditions of Ganoderma sessile and preparation of mycelium-based composite materials

  • ZHONG Zijie ,
  • LI Xueling ,
  • HU Wenfeng ,
  • YANG Meiyan
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  • 1(College of Food Science, South China Agricultural University, Guangzhou 510642, China)
    2(Shenzhen My-Loop Biotechnology Co.Ltd., Shenzhen 518122, China)

Received date: 2023-12-08

  Revised date: 2024-02-08

  Online published: 2024-12-17

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Abstract

In this study, the mycelia of Ganoderma sessile were used to prepare mycelium base composites (MBCs), C-MBCs (corn stalks), and H-MBCs (hemp stalks), respectively.To obtain more active mycelium seeds, the liquid cultivation conditions were optimized.The study included the effects of carbon sources, nitrogen sources, initial pH, temperature, G.sessile content, liquid volume, and rotating speed on the mycelial biomass of fermented products.Both C-MBCs and H-MBCs were measured for scanning electron microscope, compressive strength, and thermogravimetric characteristics.Results showed that the best mycelium cultivation medium was 25 g/L corn flour, 14 g/L soybean powder, 3 g/L KH2PO4, and 3 g/L 7H2O·MgSO4.The optimized fermentation conditions were fermentation temperature at 28 ℃, initial pH 5.5, G.sessile content with 5%, liquid volume 150 mL/500 mL flask, and rotating speed 180 r/min.Under the optimized conditions, the mycelial biomass in the cultural broth was 18.73 g/L, which increased by 34%, compared with those before optimization.The compressive strength and thermogravimetric characteristics of C-MBCs and H-MBCs were both better than expanded polystyrene.It’s concluded that this result will provide a reference for the application of MBCs instead of foam plastics in cushioning packaging.

Key words： Ganoderma sessile; mycelium; cultural medium optimization; fermentation conditions optimization; mycelium composites; biodegradable

Cite this article

ZHONG Zijie , LI Xueling , HU Wenfeng , YANG Meiyan . Optimization of mycelium liquid-state cultivation conditions of Ganoderma sessile and preparation of mycelium-based composite materials[J]. Food and Fermentation Industries, 2024 , 50(22) : 278 -285 . DOI: 10.13995/j.cnki.11-1802/ts.038205

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