Food and Fermentation Industries >

2024 , Vol. 50 >Issue 22: 334 - 338

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039088

Identification of natural lycopene based on hydrogen isotope characterization

  • LUO Jingyi ,
  • WU Zhuying ,
  • FENG Di ,
  • WANG Daobing ,
  • FU Yanmei ,
  • AN Hongmei ,
  • YANG Qingshan ,
  • LIU Yang ,
  • YUE Hongwei ,
  • ZHONG Qiding ,
  • XIE Zhoujie
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  • 1(College of Biological Engineering,Tianjin University of Science and Technology, Tianjin 300457, China)
    2(Technology Innovation Center of Light Industrial Consumer Goods Quality and Safety for State Market Regulation, Beijing 100015, China)
    3(Sinolight Technology Innovation Centre Co.Ltd., Beijing 100015, China)
    4(Hebei Chenguang Detection Technology Service Co.Ltd., Handan 300138, China)

Received date: 2024-03-05

  Revised date: 2024-05-15

  Online published: 2024-12-17

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Abstract

This study aimed to investigate the feasibility of the application of stable hydrogen isotopes in the field of lycopene adulteration detection.The method using the technique of temperature conversion/elementary analyzer-stable isotope ratio mass spectrometers (TC/EA-IRMS) was developed for the determination of stable hydrogen isotopes of lycopene from different sources.It was verified that the purity of lycopene samples was ≥94.14% after pretreatment, and the pretreatment process would not change the determination of lycopene hydrogen stable isotope ratio.The standard deviation of the δ2H value of the method was 1.99‰ for repeatability and the standard deviation of the reproducibility was less than 3‰.The stable hydrogen isotope ratios of 13 lycopene raw materials and lycopene standards were determined, the δ2H values of the synthetic lycopene ranged from -29.21‰--56.22‰, the δ2H values of the lycopene from natural sources ranged from -225.76 ‰--256.98 ‰, and the δ2H values of the lycopene from fermentation sources ranged from -81.35 ‰--141.24 ‰.The stabilized hydrogen isotope ratios of the three are significantly different, and the method can be used for the identification of lycopene from different sources.

Key words： temperature conversion/elementary analyzer-stable isotope ratio mass spectrometry (TC/EA-IRMS); lycopene; δ2H; authenticity; lycopene raw material

Cite this article

LUO Jingyi , WU Zhuying , FENG Di , WANG Daobing , FU Yanmei , AN Hongmei , YANG Qingshan , LIU Yang , YUE Hongwei , ZHONG Qiding , XIE Zhoujie . Identification of natural lycopene based on hydrogen isotope characterization[J]. Food and Fermentation Industries, 2024 , 50(22) : 334 -338 . DOI: 10.13995/j.cnki.11-1802/ts.039088

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