Food and Fermentation Industries >

2024 , Vol. 50 >Issue 24: 9 - 17

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038592

Construction of lactic acid extraction supporting liquid membrane with application to liquid cultivation of lactic acid bacteria

  • SONG Xiaoning ,
  • JIANG Jingya ,
  • ZENG Jianhua ,
  • WANG Song ,
  • ZHANG Lanwei ,
  • GONG Pimin
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  • 1(College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China)
    2(Inner Mongolia National Center of Technology Innovation for Dairy, Hohhot 150100, China)
    3(Qingdao Shangde Biotech Co.Ltd., Qingdao 266000, China)

Received date: 2024-01-15

  Revised date: 2024-02-06

  Online published: 2024-12-30

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Abstract

Utilisation of alkali to eliminate lactic acid (LA) stress during lactic acid bacteria (LAB) culturing is an important way to achieve a high-density culture of LAB, but excess alkali also causes cell death due to osmotic pressure.Hence, in situ isolation of LA during cultivation is a promising method without salt stress.In this study, the feasibility of using supported liquid membrane in situ extraction of LA to achieve a high-density culture of Lactobacillus delbrueckii ssp.bulgaricus sp1.1 was evaluated.Firstly, a supported liquid membrane extraction culture system was constructed using a polyvinylidene difluoride (PVDF) membrane as a solid support membrane, natural deep eutectic solvents (NADES) composed of food source components as a carrier, and NaOH as the receiving phase.Results showed that the 30% oleic acid/menthol (2∶1) supported liquid membrane had the best growth promotion effect of L.bulgaricus sp1.1, and produced 4.62-fold live bacteria numbers than that without extraction.Extraction of LA was achieved by the strong hydrogen bonds between the carboxyl groups of oleic acid and the hydroxyl or carboxyl groups of LA, while menthol was responsible for the stabilisation of NADES.SLM extraction cultures allow continuous growth of L.bulgaricus sp1.1 by maintaining a stable pH above 4.3 (fully inhibited pH of 4.2) compared to normal cultures.However, membrane stability issues prevented the long-term growth of L.bulgaricus sp1.1.The NADES solvent extraction system has the potential to be used in the development of high-density cultures of LAB.

Key words： lactic acid bacteria; high-density culture; lactic acid extraction; lactic acid inhibition; supported liquid membrane

Cite this article

SONG Xiaoning , JIANG Jingya , ZENG Jianhua , WANG Song , ZHANG Lanwei , GONG Pimin . Construction of lactic acid extraction supporting liquid membrane with application to liquid cultivation of lactic acid bacteria[J]. Food and Fermentation Industries, 2024 , 50(24) : 9 -17 . DOI: 10.13995/j.cnki.11-1802/ts.038592

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