Neomycin is an aminoglycoside antibiotic mainly produced by Streptomyces fradiae.It is widely used in medicine, animal husbandry and other fields.It can effectively control and treat diseases caused by bacteria, and improve the growth speed and production performance of livestock and poultry.To improve the production of neomycin, the transcriptome data of the original Streptomyces fradiae SF-1 and the mutagenic high-yield strain SF-2 were analyzed by comparative transcriptomics, and the transcription factors with significant differences in transcription levels were mined, and then the recombinant plasmids were constructed by overexpression, and then the engineered strains were constructed by conjugation transfer.The engineering strain with significant effect on neomycin synthesis was screened by shake flask fermentation, and the high-yield neomycin producing engineering strain SF-2/pP-NeyR was finally obtained by promoter optimization.Then the components of fermentation medium were orthogonal optimized, and the final titer of neomycin B was 11 843 U/mL.After comprehensive optimization, the titer of neomycin B increased by 29.6% compared with the initial one.The experimental results laid a foundation for the follow-up study of metabolic mechanism, the study of its interaction with Streptomyces fradiae neomycin synthesis gene cluster, the analysis and regulation of neomycin synthesis mechanism, and provided ideas for further improving the potency of neomycin.
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