Food and Fermentation Industries >

2025 , Vol. 51 >Issue 1: 26 - 31

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038661

Isolation and purification of cordycepin from the fermentation of engineering Yarrowia lipolytica

  • SONG Liping ,
  • DUAN Xiyu ,
  • WANG Chong ,
  • LU Xiangyang ,
  • TIAN Yun
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  • 1(College of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China)
    2(Yuanjiang Luxiaomei Food Co.Ltd., Yiyang 413000, China)

Received date: 2024-01-22

  Revised date: 2024-03-16

  Online published: 2025-01-23

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Abstract

Multiple studies have demonstrated that using Yarrowia lipolytica as a cell factory for cordycepin biosynthesis is an effective way to produce cordycepin.To obtain the separation and purification process, this study utilized macroporous adsorption resins with different specifications to purify cordycepin from recombinant Y.lipolytica fermentation broth by static and dynamic purification.Results showed that the recovery rate and purity of cordycepin could reach 87.67% and 65.82% by using NKA-Ⅱ macroporous adsorption resin with a cordycepin concentration of 3 mg/mL and pH of 9, loading flow rate of 3 BV/h, loading volume of 10 BV, wash flow rate of 2 BV/h, wash volume of 3 BV, and 30% ethanol for elution solution, with a flow rate of 3 BV/h and volume of 15 BV.This study aims to provide data references for the future large-scale production and preparation of cordycepin using recombinant Y.lipolytica.

Key words： cordycepin; Yarrowia lipolytica; separation and purification; macroporous adsorption resin; molasses

Cite this article

SONG Liping , DUAN Xiyu , WANG Chong , LU Xiangyang , TIAN Yun . Isolation and purification of cordycepin from the fermentation of engineering Yarrowia lipolytica[J]. Food and Fermentation Industries, 2025 , 51(1) : 26 -31 . DOI: 10.13995/j.cnki.11-1802/ts.038661

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