Food and Fermentation Industries >

2025 , Vol. 51 >Issue 1: 116 - 123

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038443

Process optimization of fermented brown cheese and study of its protein degradation at different stages of ripening

  • MA Xiaorui ,
  • WENG Yanan ,
  • CHEN Tao ,
  • WEI Chaokun
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  • 1(College of Food Science and Engineering, Ningxia University, Yinchuan 750021, China)
    2(Ningxia Key Laboratory of Food Microorganism Application Technology and Safety Control, College of Food Science and Engineering, Ningxia University, Yinchuan 750021, China)

Received date: 2024-01-02

  Revised date: 2024-01-18

  Online published: 2025-01-23

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Abstract

In order to develop new cheese varieties and to investigate the effect of the Maillard reaction on the physicochemical indexes and protein degradation during the fermentation of fermented brown cheese.The experimental process conditions of fermented brown cheese were optimized by single factor and response surface methodology, and the physicochemical indexes, protein degradation, free amino acid, and protease activity of fermented brown cheeses of different ripening stages were determined.The final results showed that the optimum process conditions for fermented brown cheese were:glucose addition of 10 %, heating temperature of 95 ℃, and heating time of 3.0 h.The experimental results showed that the pH and moisture of fermented brown cheese gradually decreased;The amount of proteolytic degradation and the total free amino acids increased;And the protease activity and the total number of lactic acid bacteria increased and then decreased.In the middle stage of the fermentation, the moisture content, the fat content and the protein content of fermented brown cheese were lower than ordinary cheese.This was due to the denaturation of proteins and fat globule proteins as a result of the Maillard reaction.The fat that had lost its globule membrane was destabilized and coagulates, resulting in a decrease in fat content.

Key words： Maillard reaction; fermented brown cheese; protein degradation; physical and chemical indexes; enzyme activity

Cite this article

MA Xiaorui , WENG Yanan , CHEN Tao , WEI Chaokun . Process optimization of fermented brown cheese and study of its protein degradation at different stages of ripening[J]. Food and Fermentation Industries, 2025 , 51(1) : 116 -123 . DOI: 10.13995/j.cnki.11-1802/ts.038443

References

[1] SUN Y F, LIN L, ZHANG P Y.Color development kinetics of Maillard reactions[J].Industrial & Engineering Chemistry Research, 2021, 60(9):3495-3501.
[2] GAO Y B, MIAO J J, LAI K Q.Study on Maillard reaction mechanism by quantum chemistry calculation[J].Journal of Molecular Modeling, 2023, 29(3):81.
[3] WU Y J, MAO X Q, LU J H, et al.Dairy trade helps to alleviate global carbon emission pressure[J].Environmental Science & Technology, 2022, 56(17):12656-12666.
[4] ZHANG R, JIA W.Brown goat yogurt:Metabolomics, peptidomics, and sensory changes during production[J].Journal of Dairy Science, 2023, 106(3):1712-1733.
[5] AIDARBEKOVA S, AIDER M.Production of Ryazhenka, a traditional Ukrainian fermented baked milk, by using electro-activated whey as supplementing ingredient and source of lactulose[J].Food Bioscience, 2022, 46:101526.
[6] FEENEY E L, LAMICHHANE P, SHEEHAN J J.The cheese matrix:Understanding the impact of cheese structure on aspects of cardiovascular health—A food science and a human nutrition perspective[J].International Journal of Dairy Technology, 2021, 74(4):656-670.
[7] GONÇALVES M C, CARDARELLI H R.Changes in water mobility and protein stabilization of Mozzarella cheese made under different stretching temperatures[J].LWT, 2019, 104:16-23.
[8] BARAC M, PESIC M, ZILIC S, et al.Protein profiles and total antioxidant capacity of water-soluble and water-insoluble fractions of white brined goat cheese at different stages of ripening[J].International Journal of Food Science & Technology, 2016, 51(5):1140-1149.
[9] DI GRIGOLI A, PONTE M, BONANNO A, et al.Effects of grazing season on physico-chemical characteristics and fatty acids of nutritional interest of caciocavallo palermitano cheese[J].Animals, 2022, 12(5):544.
[10] DEMERS-MATHIEU V, ST-GELAIS D, AUDY J, et al.Effect of the low-fat Cheddar cheese manufacturing process on the viability of Bifidobacterium animalis subsp.lactis, Lactobacillus rhamnosus, Lactobacillus paracasei/casei, and Lactobacillus plantarum isolates[J].Journal of Functional Foods, 2016, 24:327-337.
[11] 杨万龄, 沈兴旺, 崔凤怡, 等.蓝纹牦牛干酪成熟特性及其风味分析[J].食品科学技术学报, 2023, 41(6):52-64.
YANG W L, SHEN X W, CUI F Y, et al.Analysis of ripening characteristics and flavor of blue style-yak milk cheese[J].Journal of Food Science and Technology, 2023, 41(6):52-64.
[12] MANE A, CIOCIA F, BECK T K, et al.Proteolysis in Danish blue cheese during ripening[J].International Dairy Journal, 2019, 97:191-200.
[13] ÇAKıR Y, ÇAKMAKÇı S.Some microbiological, physicochemical and ripening properties of Erzincan Tulum cheese produced with added black cumin (Nigella sativa L.)[J].Journal of Food Science and Technology, 2018, 55(4):1435-1443.
[14] DORRIS M R, VOSS D M, BOLLOM M A, et al.Browning index of anthocyanin-rich fruit juice depends on pH and anthocyanin loss more than the gain of soluble polymeric pigments[J].Journal of Food Science, 2018, 83(4):911-921.
[15] ZHOU M M, XU L B, ZHAO F Q, et al.Regulation of milk protein synthesis by free and peptide-bound amino acids in dairy cows[J].Biology, 2021, 10(10):1044.
[16] 李彤. 枸杞籽粕酶解物及其美拉德反应产物风味特性的分析与应用[D]. 银川:宁夏大学, 2022.
LI T. Analysis and application of enzymatic hydrolysate of Lycium barbarum seed meal and its Maillard reaction products flavor characteristics[D]. Yinchuan:Ningxia University, 2022.
[17] LEEUWENDAAL N, STANTON C, O’TOOLE P W, et al.The potential of non-starter lactic acid bacteria from Cheddar cheese to colonise the gut[J].Journal of Functional Foods, 2021, 83:104425.
[18] GIOVANELLI G, CAPPA C.5-hydroxymethylfurfural formation in bread as a function of heat treatment intensity:Correlations with browning indices[J].Foods, 2021, 10(2):417.
[19] GIROUX H J, DUPONT F, VILLENEUVE G, et al.Effect of heating milk on whey protein denaturation and cheese-making properties[J].International Dairy Journal, 2020, 111:104831.
[20] ALI H M, AHMED J A O, EL ZUBEIR I E M.The effect of pH on the rheological and physical properties of analogue pizza cheese[J].Journal of Food Science and Technology, 2023, 60(2):692-700.
[21] MOREIRA R V, COSTA M P, FRASAO B S, et al.Effect of ripening time on bacteriological and physicochemical goat milk cheese characteristics[J].Food Science and Biotechnology, 2019, 29(4):459-467.
[22] GARCÍA V, ROVIRA S, BOUTOIAL K, et al.Physicochemical, microbiological, textural and sensory changes during the ripening of pasteurised goat milk cheese made with plant coagulant (Cynara scolymus)[J].International Journal of Dairy Technology, 2016, 69(1):96-102.
[23] ARBOATTI A S, OLIVARES M L, SABBAG N G, et al.The influence of sodium chloride reduction on physicochemical, biochemical, rheological and sensory characteristics of Mozzarella cheese[J].Dairy Science & Technology, 2014, 94(4):373-386.
[24] ZIARNO M, BRYŚ J, KOWALSKA E, et al.Effect of metabolic activity of lactic acid bacteria and propionibacteria on cheese protein digestibility and fatty acid profile[J].Scientific Reports, 2023, 13(1):15363.
[25] GUO M Y, SHENG Z Y, WANG P J, et al.Effects of refrigerated storage on the functional properties of processed cheese analogue with stretchability and its mechanisms[J].International Dairy Journal, 2023, 137:105504.
[26] PINO A, PRADOS F, GALÁN E, et al.Proteolysis during the ripening of goats’ milk cheese made with plant coagulant or calf rennet[J].Food Research International, 2009, 42(3):324-330.
[27] KARACA O B, GÜVEN M.Effects of proteolytic and lipolytic enzyme supplementations on lipolysis and proteolysis characteristics of white cheeses[J].Foods, 2018, 7(8):125.
[28] WANG Y W, ZHAO P Y, ZHOU Y, et al.From bitter to delicious:Properties and uses of microbial aminopeptidases[J].World Journal of Microbiology & Biotechnology, 2023, 39(3):72.
[29] GASPARD S J, AUTY M A E, KELLY A L, et al.Isolation and characterisation of κ-casein/whey protein particles from heated milk protein concentrate and role of κ-casein in whey protein aggregation[J].International Dairy Journal, 2017, 73:98-108.
[30] SINGH H, WAUNGANA A.Influence of heat treatment of milk on cheesemaking properties[J].International Dairy Journal, 2001, 11(4-7):543-551.
[31] LI L, PEI Y L, CHENG K W, et al.Production and evaluation of enzyme-modified cheese adding protease or lipase to improve quality properties[J].Journal of Bioscience and Bioengineering, 2023, 135(5):389-394.
[32] YANACHKINA P, DOOLAN I, LAPOINTE G, et al.Matching starter phenotype to functionality for low salt Cheddar cheese production based on viability, permeability, autolysis, enzyme accessibility and release in model systems[J].International Dairy Journal, 2020, 105:104682.
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