Ferulic acid is a natural compound, and its effects on liver cells are not fully understood.The aim of this study was to investigate the potential repair mechanism of ferulic acid at different concentrations on ethanol-induced DNA damage in LO2 hepatocytes.The effects of ferulic acid on the proliferation of LO2 hepatocytes were analyzed by using different concentrations of ferulic acid.Further, the effects of ethanol on LO2 cell cycle and the intervention of ferulic acid were studied.Western blot was used to detect the expression of p-H2AX, a marker of DNA damage, and the changes of reactive oxygen species levels and autophagy were detected by flow cytometry.The results showed that ferulic acid can promote the proliferation of LO2 cells at low concentration, and significantly reduce the expression of ethanol-induced p-H2AX and reactive oxygen species levels at medium concentration (0.25-2 mmol/L), and may promote autophagy.However, high concentrations of ferulic acid have shown toxic effects on cells, with potential inhibitory effects on DNA repair and autophagy.This study shows that ferulic acid can counteract ethanol-induced DNA damage in LO2 hepatocytes in a specific concentration range and may enhance the repair capacity of cells by promoting the autophagy mechanism.However, at high concentrations, ferulic acid may reverse its protective effect and show cytotoxicity.
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