Food and Fermentation Industries >

2025 , Vol. 51 >Issue 2: 25 - 34

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038510

Effect of over-expression of multicopper oxidase and glyceraldehyde 3-phosphate dehydrogenase gene on histamine degradation by Lactiplantibacillus plantarum

  • DU Xiaoyi ,
  • XU Yujuan ,
  • WU Jijun ,
  • YU Yuanshan ,
  • PENG Jian ,
  • YANG Jiguo ,
  • LI Lu
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  • 1(College of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China)
    2(Sericultural & Argi-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China)
    3(Heyuan Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Heyuan 517000, China)

Received date: 2024-01-08

  Revised date: 2024-03-04

  Online published: 2025-02-14

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Abstract

Lactiplantibacillus plantarum is one of the common strains that degrade histamine in fermented foods, its key genes for histamine degradation have not been clarified, hindering the molecular breeding of L.plantarum with a high histamine degrading ability.To reveal the key genes for histamine degradation in L.plantarum, an L.plantarum with histamine degradation ability was screened from fermented vegetables, and its strain characterization and whole genome analysis were performed.Then the key histamine degradation genes of L.plantarum were mined through over-expression.Results showed that the maximum histamine degradation rate (20.16%) of the selected strain was obtained at the 48th hour.Morphological and molecular biological characterization results exhibited that the selected was L.plantarum and named L.plantarum CY087.L.plantarum CY087 had a good tolerance to high histamine content coupled with bad tolerances to low acid and high salt.Over-expression of the multicopper oxidase gene (mcO) and the glyceraldehyde 3-phosphate dehydrogenase gene (gapdH) in L.plantarum CY087 increased its histamine degradation rate.Compared with the strain CY087-36e, the histamine degradation rates of CY087-gapdH and CY087-mcO were increased by 31.94% and 16.14%, respectively, suggesting that the gapdH gene played a more critical role in the degradation of histamine in L.plantarum CY087.This study provides potential targets for the molecular modification of L.plantarum with a high histamine degradation ability.

Key words： strain screening; Lactiplantibacillus plantarum; histamine degradation; over-expression

Cite this article

DU Xiaoyi , XU Yujuan , WU Jijun , YU Yuanshan , PENG Jian , YANG Jiguo , LI Lu . Effect of over-expression of multicopper oxidase and glyceraldehyde 3-phosphate dehydrogenase gene on histamine degradation by Lactiplantibacillus plantarum[J]. Food and Fermentation Industries, 2025 , 51(2) : 25 -34 . DOI: 10.13995/j.cnki.11-1802/ts.038510

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