Structural and physicochemical properties of starches from kernels of loquat fruits

  • MAO Qihui ,
  • WU Yuchen ,
  • LUO Qingling ,
  • LIU Ying ,
  • CHEN Jia ,
  • LEI Lin ,
  • ZHAO Guohua ,
  • YE Fayin
Expand
  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China)

Received date: 2024-02-03

  Revised date: 2024-03-14

  Online published: 2025-02-14

Abstract

Loquat is widely cultivated in subtropical regions.Current research mainly focuses on the medicinal and edible development of the leaves and fruits.However, as a processing waste, little research has been conducted on the loquat kernel and its main component starch.In this scenario, the loquat kernel starches from two main loquat varieties (Dahongpao and Dawuxing) were extracted to analyze the structure and physicochemical properties.Results showed that the extraction yields of loquat kernel starch were 23.97% and 24.64% (expressed based on fresh weight), respectively.The starch granules showed a certain particle size distribution (D50=10.70 μm, 12.00 μm;Span=0.93, 1.31) and morphology diversity, with the large granules being spherical or hemispherical with a smooth surface, and the smaller granules being ellipsoidal or polygonal.The degrees of branching (DB) of loquat kernel starch were 2.82% and 2.83%.Furthermore, the amylose of loquat kernel starch had hAm,i=0.54, 0.55, βAm,i=5.40×10-4, 5.45×10-4, hAm,ii=0.58, 0.60, and βAm,ii=18.05×10-4, 18.85×10-4.The kernel starches from two varieties of loquat had amylose content of 17.62% and 18.83%, with no significant difference in chain length distribution.The loquat kernel starch crystal type was CA type, with relative crystallinity of 26.98% and 28.40%.The thickness of semi-crystalline lamellas (dBragg) was 9.236 nm, which was a surface fractal (Ds).The physicochemical parameters such as oil binding capacity, gelatinization enthalpy (ΔHg) and the springiness of gels were not significantly different between the two starches.However, in comparison to Dawuxing, the starch from Dahongpao had higher solubility, swelling power, and greater gumminess and resilience of gels, while the peak pasting temperature (Tp), peak viscosity, hardness and adhesiveness of gels were lower.In addition, the resistant starch content of natural loquat kernel starch granules was above 94%.This study may provide experimental references for the potential applications of loquat kernel starch in starch gel foods and anti-digestive biologically active food ingredients.

Cite this article

MAO Qihui , WU Yuchen , LUO Qingling , LIU Ying , CHEN Jia , LEI Lin , ZHAO Guohua , YE Fayin . Structural and physicochemical properties of starches from kernels of loquat fruits[J]. Food and Fermentation Industries, 2025 , 51(2) : 41 -51 . DOI: 10.13995/j.cnki.11-1802/ts.038824

References

[1] MATHIOT C, PONGE P, GALLARD B, et al.Microalgae starch-based bioplastics:Screening of ten strains and plasticization of unfractionated microalgae by extrusion[J].Carbohydrate Polymers, 2019, 208:142-151.
[2] MIAO W B, WU Z W, JIANG J H, et al.The physicochemical properties of starches isolated from defatted tigernut meals:Effect of extrusion pretreatment[J].Carbohydrate Polymers, 2022, 298:120152.
[3] 谢碧霞, 陈训.中国木本淀粉植物[M].北京:科学出版社, 2008.
XIE B X, CHEN X.Woody Starch Plants of China[M].Beijing:Science Press, 2008.
[4] GUO K, LIN L S, FAN X X, et al.Comparison of structural and functional properties of starches from five fruit kernels[J].Food Chemistry, 2018, 257:75-82.
[5] SANTOS G P, MIRANDA B M, DI-MEDEIROS M C B, et al.The potential exploitation of the Malay-red apple (Syzygium malaccense) seed as source of a phosphorylated starch[J].Carbohydrate Research, 2024, 535:109008.
[6] TOSIF M M, BAINS A, SADH P K, et al.Loquat seed starch—Emerging source of non-conventional starch:Structure, properties, and novel applications[J].International Journal of Biological Macromolecules, 2023, 244:125230.
[7] COSTA B P, CARPINÉ D, DA SILVA BAMBIRRA ALVES F E, et al.Thermal, structural, morphological and bioactive characterization of acid and neutral modified loquat (Eriobotrya japonica Lindl.) seed starch and its by-products[J].Journal of Thermal Analysis and Calorimetry, 2022, 147(12):6721-6737.
[8] TUROLA BARBI R C, TEIXEIRA G L, HORNUNG P S, et al.Eriobotrya japonica seed as a new source of starch:Assessment of phenolic compounds, antioxidant activity, thermal, rheological and morphological properties[J].Food Hydrocolloids, 2018, 77:646-658.
[9] KONG X L, YANG W D, ZUO Y M, et al.Characteristics of physicochemical properties, structure and in vitro digestibility of seed starches from five loquat cultivars[J].International Journal of Biological Macromolecules, 2023, 253:126675.
[10] 方修贵, 俞国伟, 曹雪丹, 等.高直链枇杷核淀粉的提取与表征[J].食品研究与开发, 2021, 42(15):84-91.
FANG X G, YU G W, CAO X D, et al.Extraction and characterization of high amylose content starch from loquat kernels[J].Food Research and Development, 2021, 42(15):84-91.
[11] 许佳玉, 黄佳瑜, 薛岩伟, 等.枇杷核抗性淀粉制备工艺优化及其性质测定[J].食品安全质量检测学报, 2022, 13(12):4043-4050.
XU J Y, HUANG J Y, XUE Y W, et al.Optimization of preparation technology and determination of properties of loquat kernel resistant starch[J].Journal of Food Safety and Quality, 2022, 13(12):4043-4050.
[12] CAO T L, SONG K B.Effects of gum karaya addition on the characteristics of loquat seed starch films containing oregano essential oil[J].Food Hydrocolloids, 2019, 97:105198.
[13] COSTA B P, CARPINÉ D, IKEDA M, et al.Bioactive coatings from non-conventional loquat (Eriobotrya japonica Lindl.) seed starch to extend strawberries shelf-life:An antioxidant packaging[J].Progress in Organic Coatings, 2023, 175:107320.
[14] NIE M Z, PIAO C H, WANG A X, et al.Physicochemical properties and in vitro digestibility of highland barley starch with different extraction methods[J].Carbohydrate Polymers, 2023, 303:120458.
[15] VILAPLANA F, GILBERT R G.Two-dimensional size/branch length distributions of a branched polymer[J].Macromolecules, 2010, 43(17):7321-7329.
[16] CAVE R A, SEABROOK S A, GIDLEY M J, et al.Characterization of starch by size-exclusion chromatography:The limitations imposed by shear scission[J].Biomacromolecules, 2009, 10(8):2245-2253.
[17] LI Z H, WU Z H, MO G, et al.A small-angle X-ray scattering station at Beijing synchrotron radiation facility[J].Instrumentation Science & Technology, 2014, 42(2):128-141.
[18] ZHANG Y J, ZHU K X, HE S Z, et al.Characterizations of high purity starches isolated from five different jackfruit cultivars[J].Food Hydrocolloids, 2016, 52:785-794.
[19] PAES S S, YAKIMETS I, MITCHELL J R.Influence of gelatinization process on functional properties of cassava starch films[J].Food Hydrocolloids, 2008, 22(5):788-797.
[20] CHEN X T, LI X, MAO X H, et al.Study on the effects of different drying methods on physicochemical properties, structure, and in vitro digestibility of Fritillaria thunbergii Miq. (Zhebeimu) flours[J].Food and Bioproducts Processing, 2016, 98:266-274.
[21] MORALES-TREJO F, TRUJILLO-RAMIREZ D, AGUIRRE-MANDUJANO E, et al.Ultrasound-assisted extraction of lychee (Litchi chinensis Sonn.) seed starch:Physicochemical and functional properties[J].Starch-Stärke, 2022, 74(1-2):2100092.
[22] LI H T, DHITAL S, FLANAGAN B M, et al.Amorphous packing of amylose and elongated branches linked to the enzymatic resistance of high-amylose wheat starch granules[J].Carbohydrate Polymers, 2022, 295:119871.
[23] LI C, GONG B, HU Y M, et al.Combined crystalline, lamellar and granular structural insights into in vitro digestion rate of native starches[J].Food Hydrocolloids, 2020, 105:105823.
[24] ZHAO Y T, HENRY R J, GILBERT R G.Starch structure-property relations in Australian wild rices compared to domesticated rices[J].Carbohydrate Polymers, 2021, 271:118412.
[25] NADA S S, ZOU W, LI C, et al.Parameterizing amylose chain-length distributions for biosynthesis-structure-property relations[J].Analytical and Bioanalytical Chemistry, 2017, 409(29):6813-6819.
[26] CORREIA P, CRUZ-LOPES L, BEIRAO-DA-COSTA L.Morphology and structure of chestnut starch isolated by alkali and enzymatic methods[J].Food Hydrocolloids, 2012, 28(2):313-319.
[27] HE W, WEI C X.Progress in C-type starches from different plant sources[J].Food Hydrocolloids, 2017, 73:162-175.
[28] HUONG N T M, HOA P N, HUNG P V.Varying amylose contents affect the structural and physicochemical characteristics of starch in mung bean[J].International Journal of Food Properties, 2021, 24(1):737-748.
[29] FERRAZ C A, FONTES R L S, FONTES-SANT’ANA G C, et al.Extraction, modification, and chemical, thermal and morphological characterization of starch from the agro-industrial residue of mango (Mangifera indica L.) var.Uba[J].Starch-Starke, 2019, 71(1-2):1800023.
[30] CHOUDHARY P, DEVI T B, DAWANGE S P, et al.Valorization of mango by-products:Extraction and characterization of starch from seed kernels[J].Starch-Starke, 2023:2200262.
[31] YANG Z, GU Q F, LAM E, et al.In situ study starch gelatinization under ultra-high hydrostatic pressure using synchrotron SAXS[J].Food Hydrocolloids, 2016, 56:58-61.
[32] BHAT F M, RIAR C S.Effect of amylose, particle size & morphology on the functionality of starches of traditional rice cultivars[J].International Journal of Biological Macromolecules, 2016, 92:637-644.
[33] SINGH N, KAUR L.Morphological, thermal, rheological and retrogradation properties of potato starch fractions varying in granule size[J].Journal of the Science of Food and Agriculture, 2004, 84(10):1241-1252.
[34] LIU S Y, YUAN T Z, WANG X Y, et al.Behaviors of starches evaluated at high heating temperatures using a new model of Rapid Visco Analyzer-RVA 4800[J].Food Hydrocolloids, 2019, 94:217-228.
[35] IRANI M, RAZAVI S M A, ABDEL-AAL E S M, et al.Viscoelastic and textural properties of canary seed starch gels in comparison with wheat starch gel[J].International Journal of Biological Macromolecules, 2019, 124:270-281.
[36] ZHANG Y J, ZHANG Y T, XU F, et al.Molecular structure of starch isolated from jackfruit and its relationship with physicochemical properties[J].Scientific Reports, 2017, 7(1):13423.
[37] 林国荣, 张丽芬, 黄晓玲.微波处理对枇杷核淀粉理化性质和消化性能的影响[J].食品工业科技, 2023, 44(15):55-61.
LIN G R, ZHANG L F, HUANG X L.Effects of microwave treatment on the physicochemical and digestive properties of loquat kernel starch[J].Science and Technology of Food Industry, 2023, 44(15):55-61.
[38] LIN L S, HUANG J, ZHAO L X, et al.Effect of granule size on the properties of lotus rhizome C-type starch[J].Carbohydrate Polymers, 2015, 134:448-457.
Outlines

/