In the proliferation culture of Bifidobacterium, despite sufficient nutrients, the growth is found to be constrained, emphasizing the crucial exploration of its division cycle.This study focused on Bifidobacterium longum subsp.infantis B8762, employing fluorescent probes to label bacterial cells and utilizing flow cytometry to investigate the division process and membrane integrity under different temperatures.Results demonstrated that, when labelled with 10 μmol/L fluorescent dye, the strain exhibited robust growth conditions, meeting the criteria for division process assessment.Moreover, variations in the distribution of cell populations across different generations under various culture conditions indicated ongoing bacterial division, with asynchronous division among different generations.Notably, at elevated temperatures, the population of the 6th generation increased by 142.31% compared to 37 ℃.The physiological state of the strain was significantly influenced by the cultivation temperature, as evidenced by a 168.82% increase in membrane integrity loss at higher temperatures.This research unveils the fluorescent signal changes and cellular activity differences during the proliferation and division process of Bifidobacterium, providing theoretical guidance for the industrial production of highly active lactic acid bacteria.
YANG Zhan
,
YAO Guoqiang
,
LIU Kailong
,
ZHANG Xiaoyuan
,
PEI Qilin
,
LI Min
,
ZHANG Heping
. Effect of culture temperature on division cycle of Bifidobacterium longum subsp. infantis B8762[J]. Food and Fermentation Industries, 2025
, 51(2)
: 52
-58
.
DOI: 10.13995/j.cnki.11-1802/ts.038658
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