Protein is a relatively stable macromolecular substance existing in honey, and the research on honey protein is of great significance in exploring honey biological activity and screen marker ingredients.Jujube (Ziziphus jujuba Mill.) honey, a characteristic monofloral honey in China, was selected to investigate its protein components by gel-based and gel-free proteomics.Results showed that 216 bee-derived and 595 plant-derived proteins were identified in the collected samples.Among these proteins, 24 bee-derived proteins were first reported in jujube honey, including MAGUK p55 subfamily member 6 isoform X1, chitin-binding type-2 domain-containing protein, peptidase S1 domain-containing protein, and so on.Notably, by BLAST analysis, 11 proteins, including metalloendoproteinase 2-MMP-like, FAS1 domain-containing protein, peptidase S1 domain-containing protein, etc.could be considered as potential markers for jujube honey.GO analysis revealed these bee-derived and plant-derived proteins mainly were involved in the processes of carbohydrate metabolic and proteolysis, and were associated with the functions of ATP binding and guanosine triphosphate binding.The research comprehensively investigated the jujube protein components, and provided a theoretical reference for investigating the protein bioactive components and screening unique protein makers in jujube honey.
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