Food and Fermentation Industries >

2025 , Vol. 51 >Issue 2: 142 - 150

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038759

Effect of ultrasound on the functional and structural properties of cold-pressed peanut cake protein

  • YANG Chenxu ,
  • XU Yongjun ,
  • XIAO Ronghui ,
  • ZHANG Lifen ,
  • ZHU Tingwei ,
  • CHEN Fusheng ,
  • ZHANG Xianzhong
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  • 1(School of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China)
    2(Yihai Kerry (Qingdao) Grain and Oil Industry Co.Ltd., Qingdao 266322, China)
    3(School of Life Science and Engineering, Handan University, Handan 056006, China)

Received date: 2024-01-29

  Revised date: 2024-03-08

  Online published: 2025-02-14

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Abstract

In this study, cold-pressed peanut cake was taken as the research target, and the effects of ultrasound time, temperature, and power density on the structural and functional properties of cold-pressed peanut cake protein were investigated.The results showed that ultrasound treatment significantly improved the solubility, emulsifying activity, oil holding capacity, foaming capacity, and foam stability of peanut cake protein.Through ultrasound treatment at 40 min, 25 ℃ and 5.33 W/cm3, the emulsifying activity and solubility of peanut cake protein were increased by 61.05% and 49.93% respectively.The structural properties results indicated that ultrasound increased the α-helix structure of peanut cake protein, and decreased the β-sheet and the random coil structure.Meanwhile, the maximum ultraviolet absorption wavelength of peanut cake protein was blue-shifted, and the structure of peanut cake protein was unfolded and more amino acid residues were exposed.Thus, the structure of peanut cake protein molecules became more loosely and orderly.Further, ultrasound significantly changed the fluorescence intensity of peanut cake protein, with the largest emissive wavelength being blue-shifted.The analysis of the relationship between structure and function of peanut cake protein showed that ultrasound decreased the mean particle size of peanut cake protein, the secondary structure was more ordered and the hydrophobic groups were exposed through ultrasound treatment, which improved the solubility and emulsifying activity.

Key words： ultrasound; peanut cake protein; functional properties; structural properties

Cite this article

YANG Chenxu , XU Yongjun , XIAO Ronghui , ZHANG Lifen , ZHU Tingwei , CHEN Fusheng , ZHANG Xianzhong . Effect of ultrasound on the functional and structural properties of cold-pressed peanut cake protein[J]. Food and Fermentation Industries, 2025 , 51(2) : 142 -150 . DOI: 10.13995/j.cnki.11-1802/ts.038759

References

[1] 郭曼莉, 李晓彤, 吴澎, 等.花生加工副产物的综合利用及精深加工[J].粮油食品科技, 2018, 26(3):27-31.
GUO M L, LI X T, WU P, et al.Comprehensive utilization and intensive processing of by-products during peanut processing[J].Science and Technology of Cereals, Oils and Foods, 2018, 26(3):27-31.
[2] HARIHARAN S, PATTI A, ARORA A.Functional proteins from biovalorization of peanut meal:Advances in process technology and applications[J].Plant Foods for Human Nutrition, 2023, 78(1):13-24.
[3] ZHAO T, YING P F, ZHANG Y H, et al.Research advances in the high-value utilization of peanut meal resources and its hydrolysates:A review[J].Molecules, 2023, 28(19):6862.
[4] JIAO B, SHI A M, LIU H Z, et al.Effect of electrostatically charged and neutral polysaccharides on the rheological characteristics of peanut protein isolate after high-pressure homogenization[J].Food Hydrocolloids, 2018, 77:329-335.
[5] ZHANG X X, ZUO Z Y, YU P B, et al.Rice peptide nanoparticle as a bifunctional food-grade Pickering stabilizer prepared by ultrasonication:Structural characteristics, antioxidant activity, and emulsifying properties[J].Food Chemistry, 2021, 343:128545.
[6] 邵悦, 张琪, 张程, 等.超声波对花生蛋白功能特性的影响[J].食品工业科技, 2012, 33(24):130-133.
SHAO Y, ZHANG Q, ZHANG C, et al.Effects of ultrasonic technology on functional properties of peanut proteins[J].Science and Technology of Food Industry, 2012, 33(24):130-133.
[7] O’SULLIVAN J, PARK M, BEEVERS J.The effect of ultrasound upon the physicochemical and emulsifying properties of wheat and soy protein isolates[J].Journal of Cereal Science, 2016, 69:77-84.
[8] 荆卉, 孙俊, 牟瑶瑶, 等.超声波物化效应及其对蛋白质改性的应用研究进展[J].中国食品学报, 2021, 21(6):321-330.
JING H, SUN J, MU Y Y, et al.Research advance in physicochemical effect of ultrasonic and its application in protein modification[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(6):321-330.
[9] 胡爱军, 郑捷.食品超声技术[M].北京:化学工业出版社, 2013.
HU A J, ZHENG J.Food Ultrasound Technology[M].Beijing: Chemical Industry Press, 2013.
[10] 刘昕, 金明良, 覃小丽, 等.超声处理对鹰嘴豆蛋白乳化性的影响[J].食品与发酵工业, 2018, 44(5):142-147.
LIU X, JIN M L, QIN X L, et al.Effects of ultrasonic treatment on emulsifying properties of chickpea protein[J].Food and Fermentation Industries, 2018, 44(5):142-147.
[11] HAN T L, WANG M Y, WANG Y, et al.Effects of high-pressure homogenization and ultrasonic treatment on the structure and characteristics of casein[J].LWT, 2020, 130:109560.
[12] 张艳艳, 王冰蕊, 张少可, 等.超声波对花生粕蛋白功能特性及结构特性的影响[J].中国粮油学报, 2019, 34(8):48-53.
ZHANG Y Y, WANG B R, ZHANG S K, et al.Effect of ultrasonic on the functional and structural characteristics of peanut meal protein[J].Journal of the Chinese Cereals and Oils Association, 2019, 34(8):48-53.
[13] 耿军凤, 张丽芬, 陈复生.超声波辅助提取对花生蛋白结构与功能特性的影响[J].食品研究与开发, 2020, 41(9):61-69.
GENG J F, ZHANG L F, CHEN F M.Effect of ultrasound-assisted extraction on the structure and functional properties of peanut protein[J].Food Research and Development, 2020, 41(9):61-69.
[14] 孙英杰. 超声波处理对大豆分离蛋白结构和功能性质影响研究[D].哈尔滨:东北农业大学, 2014.
SUN Y J. Study on the effect of ultrasonic treatment on the structure and functional properties of soybean protein isolate. Harbin: Northeast Agricultural University, 2014.
[15] WU Y Y, XIANG X L, LIU L, et al.Ultrasound-assisted succinylation comprehensively improved functional properties of egg white protein[J].LWT-Food Science and Technology, 2022, 171:114155.
[16] 梁晗妮. 豆类蛋白质的乳化性质及其结构—功能相关性研究[D].广州:华南理工大学, 2014.
LIANG H N. Study on emulsifying properties and structure-function correlation of bean protein. Guangzhou: South China University of Technology, 2014.
[17] RESENDIZ-VAZQUEZ J A, ULLOA J A, URÍAS-SILVAS J E, et al.Effect of high-intensity ultrasound on the technofunctional properties and structure of jackfruit (Artocarpus heterophyllus) seed protein isolate[J].Ultrasonics Sonochemistry, 2017, 37:436-444.
[18] KARBASI M, ASKARI G, MADADLOU A.Effects of acetyl grafting on the structural and functional properties of whey protein microgels[J].Food Hydrocolloids, 2021, 112(9):106443.
[19] SREERAMA Y N, SASIKALA V B, PRATAPE V M.Nutritional implications and flour functionality of popped/expanded horse gram[J].Food Chemistry, 2008, 108(3):891-899.
[20] CHEN L, ZHANG S B.Structural and functional properties of self-assembled peanut protein nanoparticles prepared by ultrasonic treatment:Effects of ultrasound intensity and protein concentration[J].Food Chemistry, 2023, 413:135626.
[21] WANG Y C, LI B L, GUO Y N, et al.Effects of ultrasound on the structural and emulsifying properties and interfacial properties of oxidized soybean protein aggregates[J].Ultrasonics Sonochemistry, 2022, 87:106046.
[22] SEBII H, KARRA S, BLECKER C, et al.Effect of sonication and succinylation on rheological properties and secondary structures of date palm pollen protein concentrate[J].Rheologica Acta, 2021, 60(9):543-551.
[23] HU H, WU J H, LI-CHAN E C Y, et al.Effects of ultrasound on structural and physical properties of soy protein isolate (SPI) dispersions[J].Food Hydrocolloids, 2013, 30(2):647-655.
[24] WANG S N, YANG J J, SHAO G Q, et al.pH-induced conformational changes and interfacial dilatational rheology of soy protein isolated/soy hull polysaccharide complex and its effects on emulsion stabilization[J].Food Hydrocolloids, 2020, 109:106075.
[25] 李滢溪. 超声构建花生分离蛋白-果胶乳液体系及其稳定机制研究[D].郑州:河南工业大学, 2023.
LI Y X.Study on ultrasound construction of peanut protein isolate-pectin emulsion system and its stabilization mechanism[D].Zhengzhou:Henan University of Technology, 2023.
[26] 王艳珍, 张园园, 纵伟.超声处理对杜仲翅果蛋白结构及功能特性的影响[J].中国油脂, 2024,49(5):66-70.
WANG Y Z, ZHANG Y Y, ZONG W.Effect of ultrasonic on the structure and functional properties of protein from Eucommia key-fruit seed[J].China Oils and Fats, 2024,49(5):66-70.
[27] WANG W, LI J S, YAN L J, et al.Effect of oxidization and chitosan on the surface activity of soy protein isolate[J].Carbohydrate Polymers, 2016, 151:700-706.
[28] DING Q Y, TIAN G F, WANG X H, et al.Effect of ultrasonic treatment on the structure and functional properties of mantle proteins from scallops (Patinopecten yessoensis)[J].Ultrasonics Sonochemistry, 2021, 79:105770.
[29] 张熙. 扫频超声波预处理对β-乳球蛋白酶解特性及聚集行为的影响机理研究[D].镇江:江苏大学, 2019.
ZHANG X. Study on the influence mechanism of swept-frequency ultrasonic pretreatment on the proteolytic characteristics and aggregation behavior of β-globule. Zhenjiang: Jiangsu University, 2019.
[30] 许英一, 张微, 王宇, 等.超声对小麦麸皮蛋白结构和功能性质的影响[J].中国粮油学报, 2023, 38(10):53-61.
XU Y Y, ZHANG W, WANG Y, et al.Effect of ultrasonic on structure and functional properties of wheat bran protein[J].Journal of the Chinese Cereals and Oils Association, 2023, 38(10):53-61.
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