In this work, chronic unpredictable mild stress (chronic unpredictable mild stress, CUMS) model mice were fed a composite powder containing Lutianus erythropterus crude protein with oligomaltose, oligofructose, and galacto-oligosaccharides (PRSP) to examine its potential to prevent depression-like behavior and organism-related injury.One hundred and eighty mice were split into four groups including an intervention group (PRSP), a model group (CUMS), a fluoxetine control group (FLU), and an empty control group (CON).For 42 days, the mice in the model group were subjected to CUMS modeling.At the same time as modeling, some animals received 1.8 g/(kg·d) PRSP via gavage, while a positive control group of mice received 10 mg/(kg·d) fluoxetine.Following the meal, behavioral evaluations, colon slice hematoxylin-eosin staining and immunohistochemical staining, serum lipopolysaccharides (LPS) levels, D-lactic acid (D-LA), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β, and IL-10, high-throughput sequencing, and bioinformatic analysis of colon content samples were performed in mice of each group.Mouse models subjected to CUMS modeling exhibited aberrant behavioral indices, higher colonic damage scores, markedly reduced expression of intestinal epithelial mucin-2 (MUC2), and markedly raised blood levels of D-LA, LPS, TNF-α, IL-1β, and IL-6.The CUMS group experienced a significant decrease in the number of taxonomic units unique to the contents of the colon.The Firmicutes had a much greater abundance ratio than Bacteroidetes overall.There was a significant decrease in the abundance of Lachnospiraceae and Oscillibacter and a significant increase in the abundance of Alistipes in the colon of CUMS mice.The abundance of colony glutamate, glutamine, tyrosine, threonine, galactose, fructose, sucrose, and maltose metabolic pathway genes was significantly increased, and all of the above indices were restored to the level of the empty control group after the PRSP intervention.The compound powder could inhibit the structural disorders of the colon flora of mice caused by chronic stress and its specific abnormal increase in amino acid and glucose metabolism, prevent the occurrence of systemic inflammation and colon damage in the body, and play a good preventive role in depression-like behavior, and the results of the study can provide theoretical support for the development of efficient and safe food for the prevention of depression.
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