Water extraction and alcohol precipitation method was used to extract the polysaccharide from Elaeagnus moorcroftii Wall.of Kashgar (EMP-K).Physicochemical properties of polysaccharides were carried out through modern analytical techniques, and then protein-polyphenol-polysaccharide ternary composite emulsion system was constructed and its storage stabilities were investigated.Results showed that the EMP-K was a heteropolysaccharide with an average molecular weight of 7 491 Da, mainly composed of Man, GlcN, Rha, GlcUA, GalUA, Glc, Gal, Ara, etc., the infrared spectra showed that the samples exhibited vibrational absorption peaks of C=O and C—O, the scanning electron microscopy results showed that the apparent appearance of EMP-K exhibited a clear granular structure with a relatively smooth microsurface, the particle size and Zeta potential measurements showed that the solvent system of polysaccharides was relatively stable, the covalent reaction between lactoferrin and epigallocatechin gallate occurred in the ternary complex, and the polysaccharides were mainly bound to the binary complex through hydrogen bonding and hydrophobic interactions. Moreover, the bilayered emulsion formed with the participation of polysaccharides could effectively prevent the aggregation of droplets to enhance the storage stability of the system.This study could provide an important scientific basis for the in-depth development of emulsifying function of Elaeagnus moorcroftii Wall.polysaccharide, the enhancement of the comprehensive benefits of Elaeagnus moorcroftii Wall.in Xinjiang and the development of a new type of emulsion delivery systems.
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