Food and Fermentation Industries >

2025 , Vol. 51 >Issue 2: 293 - 299

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040507

Detection of deoxynivalenol in corn using gold nanoflowers based immunochromatographic test strips

  • WANG Zhihua ,
  • ZHAO Yiming ,
  • REN Wenjie ,
  • WANG Yulong ,
  • LI Yifan ,
  • ZENG Juntao ,
  • HE Baoshan
Expand
  • 1(National Engineering Laboratory,Provincal Key Laboratory of food Science Discipline, Henan University of Technology, Zhengzhou 450001, China)
    2(Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)

Received date: 2024-07-18

  Revised date: 2024-08-30

  Online published: 2025-02-14

Fold

Abstract

Deoxynivalenol (DON) poses a great threat to human and animal health, as well as the food economy.This study established a method for the rapid detection of DON in corn using a gold nanoflowers (AuNFs) -based immunochromatographic test strips (ICTS).AuNFs were prepared using a seed growth method and conjugated with anti-DON monoclonal antibodies to generate the gold-labeled probes for immunochromatographic test strips.The pH, antibody volume, sample buffer concentration, T-line concentration, and the volume of gold-labeled probe were optimized.The developed method was subsequently used for detecting DON in corn.After optimizing the experimental conditions, the optimal pH was determined to be 7.5, the best antibody volume was 1 μL, the most suitable sample buffer concentration was PBST+1% PVP K30, the optimal T-line concentration was 0.1 mg/mL, and the volume of gold-labeled probe was 3 μL.Under the optimal conditions of the reaction system, the method achieved a detection limit of 20 μg/kg, with a sensitivity of ≥99%, a false positive rate of ≤1%, and a false negative rate of ≤1%.Additionally, the results were consistent with existing standard methods and show no cross-reactivity with other mycotoxins.The ICTS using the AuNFs-based immunochromatographic test strips exhibits high sensitivity and strong specificity, enabling rapid detection of DON in corn.

Key words： gold nanoflowers; seed growth method; immunochromatographic test strips; rapid detection; deoxynivalenol

Cite this article

WANG Zhihua , ZHAO Yiming , REN Wenjie , WANG Yulong , LI Yifan , ZENG Juntao , HE Baoshan . Detection of deoxynivalenol in corn using gold nanoflowers based immunochromatographic test strips[J]. Food and Fermentation Industries, 2025 , 51(2) : 293 -299 . DOI: 10.13995/j.cnki.11-1802/ts.040507

References

[1] LIAO B Y, HU L L, LI H G, et al.Toxicity of the mycotoxin deoxynivalenol on early cleavage of mouse embryos by fluorescence intensity analysis[J].Microscopy and Microanalysis, 2023, 29(2):754-761.
[2] ZHANG Y L, OUYANG B B, ZHANG W L, et al.Deoxynivalenol:Occurrence, toxicity, and degradation[J].Food Control, 2024, 155:110027.
[3] VIDAL A, BOUZAGHNANE N, DE SAEGER S, et al.Human mycotoxin biomonitoring:conclusive remarks on direct or indirect assessment of urinary deoxynivalenol[J].Toxins, 2020, 12(2):139.
[4] GANESAN A R, MOHAN K, KARTHICK RAJAN D, et al.Distribution, toxicity, interactive effects, and detection of ochratoxin and deoxynivalenol in food:A review[J].Food Chemistry, 2022, 378:131978.
[5] 曾运婷, 李再新.小麦中呕吐毒素检测方法的研究进展[J].广东化工, 2023, 50(21):142-144.
ZENG Y T, LI Z X.Review on detection methods for vomiting toxins in wheat[J].Guangdong Chemical Industry, 2023, 50(21):142-144.
[6] 朱海华, 张梦雪, 胡骁飞, 等.食品中呕吐毒素检测方法的研究进展[J].食品科技, 2021, 46(11):314-320.
ZHU H H, ZHANG M X, HU X F, et al.Research progress on the detection method of vomitoxin in food[J].Food Science and Technology, 2021, 46(11):314-320.
[7] 陈冉, 邱艳, 都晓慧, 等.粮食中呕吐毒素检测技术研究[J].粮食储藏, 2023, 52(3):39-42.
CHEN R, QIU Y, DU X H, et al.Study on detection of deoxyniverenol in grain[J].Grain Storage, 2023, 52(3):39-42.
[8] WANG W, MA J J, YU C C, et al.Simultaneous determination of masked deoxynivalenol and some important type B trichothecenes in Chinese corn kernels and corn-based products by ultra-performance liquid chromatography-tandem mass spectrometry[J].Journal of Agricultural and Food Chemistry, 2012, 60(46):11638-11646.
[9] 谭林, 陆阳, 王正珅.玉米中真菌毒素检测技术的研究进展[J].粮食加工, 2023, 48(6):111-114.
TAN L, LU Y, WANG Z S.Research prograss of detection technology of mycotoxin contamination of maize[J].Grain Processing, 2023, 48(6):111-114.
[10] 刘云翔, 周荣荣, 詹志来, 等.薏苡仁中呕吐毒素酶联免疫检测方法的建立[J].中国中药杂志, 2022, 47(24):6581-6586.
LIU Y X, ZHOU R R, ZHAN Z L, et al.Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for determination of vomitoxin (DON) in Coicis Semen[J].China Journal of Chinese Materia Medica, 2022, 47(24):6581-6586.
[11] 王振芳, 王平东, 邓庆, 等.高灵敏度呕吐毒素时间分辨荧光快速定量检测卡的研发和应用[J].粮食加工, 2024, 49(1):102-107.
WANG Z F, WANG P D, DENG Q, et al.Development and application of high sensitivity time-resolved fluorescent rapid quantitative detection card for deoxynivalenol[J].Grain Processing, 2024, 49(1):102-107.
[12] 刘以晴, 黄亚伟, 王小庆, 等.粮食中真菌毒素快速检测方法研究进展[J].粮食与饲料工业, 2023(2):66-71.
LIU Y Q, HUANG Y W, WANG X Q, et al.Research progress on rapid detection methods of mycotoxins in grain[J].Cereal & Feed Industry, 2023(2):66-71.
[13] 李华英, 闫文婧, 李进春, 等.粮油中多种真菌毒素检测技术研究进展[J].农产品加工, 2023(12):67-70.
LI H Y, YAN W J, LI J C, et al.Research progress on detection technology of mycotoxins in grains and oils[J].Farm Products Processing, 2023(12):67-70.
[14] 周菊桃, 何军意, 肖国强, 等.基于纳米材料的呕吐毒素生物传感研究与应用[J].化学传感器, 2023, 43(2):8-18.
ZHOU J T, HE J Y, XIAO G Q, et al.Research and application on deoxynivalenol biosensors based on nanomaterials[J].Chemical Sensors, 2023, 43(2):8-18.
[15] WANG X Y, SUN T Q, SHEN W L, et al.A lateral flow immunochromatographic assay based on nanobody-oriented coupling strategy for aflatoxin B1 detection[J].Sensors and Actuators B:Chemical, 2023, 394:134419.
[16] WU W J, LI Y, XU Q B, et al.Polydopamine-coated HKUST MOFs-based strip lateral flow immunoassay for on-site ultrasensitive detection of aflatoxin B1 in foods[J].Food Control, 2023, 152:109864.
[17] 金玉, 刘仁荣, 裘雪梅.基于金纳米花标记的免疫层析法检测牛奶中黄曲霉毒素M1[J].食品研究与开发, 2019, 40(13):164-170.
JIN Y, LIU R R, QIU X M.Sensitive detection of aflatoxin M1 in milk by immunochromatography based on gold nanoflower labeled[J].Food Research and Development, 2019, 40(13):164-170.
[18] CHEN X R, ZHANG J Y, XIE J H, et al.Development of two immunochromatographic test strips based on gold nanospheres and gold nanoflowers for the rapid and simultaneous detection of aflatoxin B1 and aristolochic acid a in dual-use medicinal and food ingredients[J].Microchemical Journal, 2023, 186:108307.
[19] HUANG X, HUANG X Y, XIE J H, et al.Rapid simultaneous detection of fumonisin B1 and deoxynivalenol in grain by immunochromatographic test strip[J].Analytical Biochemistry, 2020, 606:113878.
[20] ZHOU S Y, XU L G, KUANG H, et al.Fluorescent microsphere immunochromatographic sensor for ultrasensitive monitoring deoxynivalenol in agricultural products[J].Microchemical Journal, 2021, 164:106024.
[21] SUN J D, WANG L Z, SHAO J D, et al.One-step time-resolved fluorescence microsphere immunochromatographic test strip for quantitative and simultaneous detection of DON and ZEN[J].Analytical and Bioanalytical Chemistry, 2021, 413(26):6489-6502.
[22] 章铜, 沈央红, 张雯, 等.基于普鲁士蓝纳米粒子的免疫层析法检测小麦中的呕吐毒素[J].食品与发酵工业, 2024, 50(4):286-293.
ZHANG T, SHEN Y H, ZHANG W, et al.Based on Prussian blue nanoparticles for immunochromatographic detection of deoxynivalenol in wheat[J].Food and Fermentation Industries, 2024, 50(4):286-293.
[23] LI R, BU T, ZHAO Y J, et al.Polydopamine coated zirconium metal-organic frameworks-based immunochromatographic assay for highly sensitive detection of deoxynivalenol[J].Analytica Chimica Acta, 2020, 1131:109-117.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.