Food and Fermentation Industries >

2025 , Vol. 51 >Issue 3: 1 - 11

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038724

Screening, preparation, and efficacy evaluation of hydrolysates from marine protein sources for sobering up and liver protection

  • FENG Yangmengxiao ,
  • REN Qingxi ,
  • LIU Shuangping ,
  • ZHOU Zhilei ,
  • JI Zhongwei ,
  • MAO Jian
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  • 1(National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China)
    2(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    3(Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Wuxi 214122, China)
    4(Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Wuxi 214122, China)
    5(Jiangnan University (Shaoxing) Industrial Technology Research Institute, Shaoxing 312000, China)
    6(National Engineering Research Center for Huangjiu, Shaoxing 312000, China)

Received date: 2024-01-26

  Revised date: 2024-04-08

  Online published: 2025-02-21

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Abstract

336 samples were prepared by double enzyme hydrolysis of 8 kinds of marine organisms.The target hydrolysate with the best comprehensive effect was screened out, which was Stichopus japonicus peptides (SJPs).It was the product of enzymatic hydrolysis of sea cucumber by alkaline protease and flavourzyme.SJPs had a high in vitro ethanol dehydrogenase activation rate (118.86%), hydrophobic amino acid content (45.97%), small peptide content (81.04%), and gastrointestinal digestive stability.The acute alcoholism model and early alcoholic liver disease model of mice were established to evaluate the anti-alcoholic and hepatoprotective activities of SJPs.Results showed that oral administration of SJPs before drinking significantly prolonged the drunken latency and shortened the sleep period of mice, enhancing the tolerance to acute alcohol toxicity.In addition, SJPs accelerated the clearance of alcohol and its metabolites by activating the alcohol metabolic enzyme system, reducing liver oxidative stress and fat accumulation, and had a significant protective effect on early liver injury.In summary, SJPs may played a potential role as a functional food ingredient to alleviate alcoholism and protect the liver.

Key words： Stichopus japonicus peptides; anti-alcohol and liver protection; double enzymatic hydrolysis; alcohol dehydrogenase (ADH) activation rate; alcoholic liver injury

Cite this article

FENG Yangmengxiao , REN Qingxi , LIU Shuangping , ZHOU Zhilei , JI Zhongwei , MAO Jian . Screening, preparation, and efficacy evaluation of hydrolysates from marine protein sources for sobering up and liver protection[J]. Food and Fermentation Industries, 2025 , 51(3) : 1 -11 . DOI: 10.13995/j.cnki.11-1802/ts.038724

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