Lactobacillus species have been recognized for their potential to modulate the antioxidant signalling pathway, thereby upregulating the expression of antioxidant enzymes and mitigating oxidative damage.The study was undertaken to explore the enhancement of host antioxidant capacity by specific probiotics and their role in modulating oxidative stress induced by Gardnerella vaginalis (GV) infection.An inflammatory model was developed by treating VK2/E6E7 cells with 1.25 μg/mL lipopolysaccharide (LPS) for 24 hours.The Cell Counting Kit-8 (CCK-8) assay was then employed to identify Lactobacilli with antioxidant potential.An animal infection model was established via subcutaneous injection of 5 mg/mL estradiol valerate in the neck and vaginal injection of 20 μL containing 1010 CFU/mL GV solution.Subsequently, a 100 μL Lactobacillus suspension at a concentration of 109 CFU/mL was gavaged to the mice daily for 10 days.At the end of the experiment, GV load, histopathological assessments, and expression levels of the antioxidant signalling pathway proteins Kelch-like ECH-associated protein 1 (Keap1) and nuclear factor erythroid 2-related factor 2 (Nrf2) in vaginal tissue of various group were evaluated.Results showed that Lactobacillus paracasei CCFM1317 significantly enhanced cell viability to 109.0% in vitro.In the animal trial, oral administration of CCFM1317 led to a reduction in the GV load by 40.6%.Histopathological examination of vaginal tissue revealed epithelial continuity restoration and a decrease in the severity of inflammatory cell infiltration.Furthermore, investigation of Keap1/Nrf2 signalling pathway expression in vaginal tissue revealed that CCFM1317 markedly decreased Keap1 mRNA expression by 48.6% and significantly increased Nrf2 mRNA expression (P<0.000 1), with a 1.9-fold increase in Nrf2 content.These findings demonstrated that L.paracasei CCFM1317 possesses characteristics that enhance vaginal antioxidant defenses and may potentially alleviate oxidative stress-related symptoms during vaginitis.
LIN Rumeng
,
HUANG Xiaoyan
,
CUI Shumao
,
MAO Bingyong
,
TANG Xin
,
ZHAO Jianxin
,
ZHANG Qiuxiang
. Lacticaseibacillus paracasei CCFM1317 alleviates Gardnerella vaginalis infection in mice by activating expression of Keap1/Nrf2 pathway[J]. Food and Fermentation Industries, 2025
, 51(3)
: 21
-27
.
DOI: 10.13995/j.cnki.11-1802/ts.039076
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