Inhibition effects of Lactiplantibacillus pentosus Z097 extract against Listeria monocytogenes quorum sensing signaling molecule and biofilm

  • YE Guoliang ,
  • ZHANG Xingguo ,
  • HUANG Mingxin ,
  • LI Ting ,
  • ZHONG Qingping
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  • (Guangdong Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China)

Received date: 2024-01-24

  Revised date: 2024-03-14

  Online published: 2025-02-21

Abstract

Listeria monocytogenes is a common foodborne pathogen.Active substances of lactic acid bacteria have potential abilities to control L.monocytogenes through quenching quorum sensing (QS) systems and inhibiting biofilm formation.In this study, the minimum inhibitory concentration (MIC) of ethyl acetate extract of Lactiplantibacillus pentosus Z097 (Z097-E) against L.monocytogenes was determined, and the effects of sub-MIC of Z097-E on the growth, auto-aggregation, hydrophobicity, motility, biofilm formation, exopolysaccharide, extracellular protein, and QS signal AI-2 of L.monocytogenes were explored.The elimination effects of different concentrations of Z097-E on mature biofilm were also measured.Results showed that the MIC of Z097-E was 6.4 mg/mL.At 3.2 mg/mL of Z097-E, the auto-aggregation rate and hydrophobicity of L.monocytogenes were decreased by 73.58% and 89.62%, respectively.Meanwhile, the inhibition rates on exopolysaccharide, extracellular proteins, and AI-2 of L.monocytogenes were 80.03%, 34.23%, and 84.43%, respectively.When treated with 3.2 mg/mL of Z097-E for 24 and 48 hours, the biofilm biomass was decreased by 52.76% and 53.77%, respectively.The elimination rates of 12.8 mg/mL of Z097-E on mature biofilm for 24 h and 48 h were 30.08% and 62.07%, respectively.Results showed that the Z097-E could inhibit the biofilm and quorum sensing of L.monocytogenes, providing a reference for the control of L.monocytogenes biofilm in food processing, transportation, and preservation processes.

Cite this article

YE Guoliang , ZHANG Xingguo , HUANG Mingxin , LI Ting , ZHONG Qingping . Inhibition effects of Lactiplantibacillus pentosus Z097 extract against Listeria monocytogenes quorum sensing signaling molecule and biofilm[J]. Food and Fermentation Industries, 2025 , 51(3) : 64 -71 . DOI: 10.13995/j.cnki.11-1802/ts.038698

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