Food and Fermentation Industries >

2025 , Vol. 51 >Issue 3: 97 - 103

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038913

Study on the antibacterial effect of two Bacillus lipopeptide crude extracts on potato Phytophthora infestans

  • HUANG Man ,
  • LI Huanmei ,
  • HU Jing ,
  • CHEN Zhiyong ,
  • WEI Junjie ,
  • LONG Tong ,
  • SONG Man ,
  • ZHAO Jinfang
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  • 1(School of Life Sciences and Health, Hubei University of Technology, Key Laboratory of Fermentation Engineering (Ministry of Education), Wuhan 430068, China)
    2(Hubei Biopesticide Engineering Research Center, Wuhan 430064, China)

Received date: 2024-02-20

  Revised date: 2024-04-10

  Online published: 2025-02-21

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Abstract

To investigate the bacteriostatic effect of crude lipopeptide extracts of Paenibacillus polymyxa HL11 and Bacillus cabrialesii ST-1 on Phytophthora infestans, the lipopeptide crude extracts of HL11 and ST-1 were extracted by acid precipitation method, and the antibacterial effect of the lipopeptide crude extracts of the two Bacillus strains on Phytophthora infesfans was determined by Oxford cup method.The results showed that the inhibition rates of HL11 and ST-1 against P.infestans were (74.79 ±0.52)% and (79.5±0.54)%, respectively, when the concentration of lipopeptide crude extract was 10 mg/mL. The EC50of the two strains of Bacillus were 0.56 and 0.021 mg/mL, respectively, as derived from the software fitting.Further observation by scanning electron microscopy showed that after treatment with lipopeptide crude extract, the surface of P.infestans showed depression, deformation or protrusions.Fluorescence microscopy showed that the structural integrity of P.infestans was destroyed to a certain extent after treatment with lipopeptide crude extract, and chromatin condensation and ROS surge occurred in the cells.By MALDI-TOF-MS analysis and data comparison, lipopeptides were mainly Iturin A, Surfactin and Fengycin.In this study, the crude lipopeptide extracts of two Bacillus strains had obvious inhibitory effect on Phytophthora infestans, and had the potential for further research and development.

Key words： Paenibacillus polymyxa HL11; Bacillus cabrialesii ST-1; lipopeptides; Phytophthora infestans; inhibitory rates

Cite this article

HUANG Man , LI Huanmei , HU Jing , CHEN Zhiyong , WEI Junjie , LONG Tong , SONG Man , ZHAO Jinfang . Study on the antibacterial effect of two Bacillus lipopeptide crude extracts on potato Phytophthora infestans[J]. Food and Fermentation Industries, 2025 , 51(3) : 97 -103 . DOI: 10.13995/j.cnki.11-1802/ts.038913

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