Ethanol, a renewable energy, relies predominantly on yeast metabolism for its production.Selecting strains exhibiting high tolerance and productivity in ethanol fermentation is the key to achieve enhanced ethanol yields.In order to select and breed high-tolerant and high-yielding ethanol yeast strains, with six existing haploid brewing yeast strains, a mutant library was constructed through atmospheric and room temperature plasma mutagenesis, which were colorimetrically screened by triphenyl tetrazolium chloride, re-screened by pressure growth at 40, 41 ℃, and quantitatively analyzed by fermentation screening medium.Subsequently, nine dominant mutant haploid strains were obtained and hybridized through different mating types, resulting in a diploid brewing yeast strain, C6.with superior fermentation efficiency, better temperature resilience, acidity tolerance, and ethanol resistance than parental strains.The results exhibited that in a 35 ℃ composite sugar water fermentation medium, the C6 strain achieved an ethanol content of 14.6%, whereas parental strains AE2 and M reached 12.82% and 13.72%, respectively.Notably, the ethanol content increased by 13.8% and 6.4%, while at 30 ℃, the ethanol content for C6 came to 17.9%.It was evident that the stable OD680 values period of the improved temperature tolerance of C6, AE2, and M strains were 4.12, 0.35, 1.15, respectively, under 40 ℃ conditions, showing a biomass increase ranging from 1.2 to 12 times.Thus, the C6 strain exhibits promising industrial applications in the field of ethanol production
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