Food and Fermentation Industries >

2025 , Vol. 51 >Issue 3: 349 - 358

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040869

Multi-walled carbon nanotubes improved QuEChERS extraction combined with HPLC-MS/MS for rapid determination of tetraniliprole residues in aquatic products

  • YUAN Liejiang ,
  • ZHANG Kexin ,
  • LI Zheng ,
  • ZHU Li ,
  • DENG Hang ,
  • WU Haizhi ,
  • CHEN Yao ,
  • XUE Minmin ,
  • SHANGGUAN Jia ,
  • WANG Xiu ,
  • LI Ge ,
  • SUN Yiqing
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  • 1(Hunan Provincial Institute of Product and Goods Quality Inspection, Changsha 410007, China)
    2(Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha 410111, China)
    3(Yueyang Inspection and Testing Center, Yueyang 410042, China)

Received date: 2024-08-28

  Revised date: 2024-10-17

  Online published: 2025-02-21

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Abstract

An analytical approach was developed for the rapid determination of tetraniliprole hydrochloride residues in aquatic products.This method involved a modified QuEChERS extraction combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and multi-walled carbon nanotubes (MWCNTs).The samples were extracted using acetonitrile, and then partitioned into aqueous and organic phases by adding 4 g of anhydrous sodium sulfate and 4 g of anhydrous magnesium sulfate.The purification process involved the use of MWCNTs-improved QuEChERS, which included the addition of 3 mg of MWCNTs, 60 mg of C18, and 50 mg of PSA to each milliliter of extraction solution.Subsequently, the samples were separated on Agilent Venusil MP C18 (100 mm×2.1 mm, 3 μm) column, eluted with a methanol-water mixture, ionized via electrospray ionization in negative ion mode, and quantified using multiple reaction monitoring (MRM) with matrix matching external standard calibration.The results showed that the relationship coefficient (R2) of tetraniliprole in different aquatic product matrices at 0.1-20 μg/L was greater than 0.999.The quantitative limit of method (S/N≥10) was 0.80 μg/kg.The recovery rates at 2.0 μg/kg, 4.0 μg/kg, and 20 μg/kg for carp, yellowtail, crayfish, grass shrimp, hairy crab, swimming crab, and clam as matrix were between 82% and 97% with relative standard deviation (RSD) of 0.66%-4.02% (n=6) for intra-batch and RSD of 0.88%-2.95% (n=6) for inter-batch.The method is convenient and efficient, and can meet the requirements for the detection of tetraniliprole residues in aquatic products.

Key words： high-performance liquid chromatography mass spectrometer/mass spectrometry hybrid instrument; QuEChERS; tetraniliprole; multi-walled carbon nanotubes; residue

Cite this article

YUAN Liejiang , ZHANG Kexin , LI Zheng , ZHU Li , DENG Hang , WU Haizhi , CHEN Yao , XUE Minmin , SHANGGUAN Jia , WANG Xiu , LI Ge , SUN Yiqing . Multi-walled carbon nanotubes improved QuEChERS extraction combined with HPLC-MS/MS for rapid determination of tetraniliprole residues in aquatic products[J]. Food and Fermentation Industries, 2025 , 51(3) : 349 -358 . DOI: 10.13995/j.cnki.11-1802/ts.040869

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