Food and Fermentation Industries >

2025 , Vol. 51 >Issue 3: 368 - 375

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039448

Research methods of enzymes and research progress of functional enzymes in Baijiu Daqu

  • YAN Cheng ,
  • ZHANG Liqiang ,
  • RAN Maofang ,
  • HUANG Zhangjun ,
  • ZENG Yunhang ,
  • SHI Bi
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  • 1(College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China)
    2(Luzhou Pinchuang Science & Technology Co.Ltd., National Engineering Research Center of Solid-state Brewing, Luzhou 646000, China)
    3(Luzhou Laojiao Co.Ltd., Luzhou 646000, China)

Received date: 2024-04-03

  Revised date: 2024-05-09

  Online published: 2025-02-21

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Abstract

Enzymes of Daqu are one of the key factors affecting the quality of Daqu and Baijiu.Understanding the enzymes is of great significance for optimizing the Daqu-making technology and revealing the mechanism of Baijiu fermentation.In this study, advanced technologies for investigating the enzymes of Daqu were introduced, and their advantages and disadvantages were discussed.Additionally, known functional enzymes in typical Daqu were summarized.High-throughput sequencing and metaproteomics could be used to analyze the composition and function of enzymes in Daqu from the perspective of genome, transcriptome, and proteome.Currently, the key functional enzymes involved in the pathways of saccharification and ethanol fermentation in low-temperature Daqu, medium-temperature Daqu, and high-temperature Daqu have been identified by using the above technologies.However, because the above technologies have difficulties in detecting enzymes with lower content in Daqu, the functional enzymes involved in the pathways of flavor compound metabolism in Daqu cannot be fully identified at present.There is an urgent need to upgrade relevant technologies to promote the research on the enzymes in Daqu.

Key words： Daqu; enzymes; functional enzymes; omics technology; metaproteomics

Cite this article

YAN Cheng , ZHANG Liqiang , RAN Maofang , HUANG Zhangjun , ZENG Yunhang , SHI Bi . Research methods of enzymes and research progress of functional enzymes in Baijiu Daqu[J]. Food and Fermentation Industries, 2025 , 51(3) : 368 -375 . DOI: 10.13995/j.cnki.11-1802/ts.039448

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