Putrescine is mainly used in the preparation of high-temperature-resistant nylon PA46.Currently, it is mainly monopolized due to the high technical barriers derived from using highly toxic hydrocyanic acid and acrylonitrile as raw materials.To address the low yield and productivity in the biological synthesis of putrescine, this research improved the substance transfer efficiency via permeabilization of cells based on a biocatalytic route from arginine to putrescine, and the synthesis ability was enhanced through engineering strain cultivation and catalytic process enhancement.The results showed that the ethanol permeabilization treatment could significantly enhance the mass transfer limitation, and the putrescine yield was enhanced by more than 50% compared with the control, reaching 14.92 g/L.Meanwhile, the optimal cultivation conditions and the catalytic process were determined, and the putrescine yield reached the highest reported level of 88.67 g/L,with a molar conversion rate 0.81 mol/mol and a production efficiency of 2.46 g/(L·h), laying the foundation for the subsequent development of biobased putrescine.
DING Bo
,
LIU Wei
,
MAO Yin
,
LI Guohui
,
DENG Yu
. Efficient synthesis of putrescine by permeabilization treatment of recombinant Escherichia coli[J]. Food and Fermentation Industries, 2025
, 51(5)
: 15
-21
.
DOI: 10.13995/j.cnki.11-1802/ts.038803
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