Food and Fermentation Industries >

2025 , Vol. 51 >Issue 5: 95 - 102

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039392

Trypsin regulates synthesis of phenylpropane compounds induced by CsWRKY33 in mitogen-activated protein kinase pathway of cucumber

  • SUN Jiaju ,
  • WANG Hemin ,
  • LI Xin
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  • (College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China)

Received date: 2024-03-28

  Revised date: 2024-04-21

  Online published: 2025-03-28

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Abstract

The mitogen-activated protein kinase (MAPK) cascade pathway extensively participates in the physiological regulation processes of plants.Trypsin, as an inducer of resistance, significantly enhances cucumber storage quality.However, the mechanism by which trypsin regulates the synthesis of phenylpropanoid compounds through the MAPK pathway to influence fruit resistance remains to be elucidated.To investigate the molecular mechanism underlying trypsin-induced cucumber resistance, this study conducted a combined transcriptomic and widely targeted metabolomic analysis, validated through virus-induced gene silencing (VIGS).Transcriptomic results revealed that a total of 83 differentially expressed genes were enriched in the MAPK pathway, with 48 genes significantly downregulated and 35 genes significantly upregulated.Gene set enrichment analysis (GSEA) further identified the CsWRKY33 transcription factor from the leading edge subset.Multiple sequence alignment results with plants such as Arabidopsis thaliana and Nicotiana tabacum showed that the transcription factor belonged to the WRKY family and had a highly conserved WRKY domain at the N-terminus.The combined analysis of transcriptomics and widely targeted metabolomics demonstrated significant upregulation in the synthesis of compounds such as vanillin, dihydrochalcone-4′-O-glucoside, and 2-hydroxy-3-phenylpropanoic acid.Co-expression network analysis suggested that these key metabolites were negatively regulated by CsWRKY33.VIGS results showed that silencing CsWRKY33 enhanced fruit resistance and extended storage time.This study revealed that trypsin could downregulate the expression of CsWRKY33, promoting the synthesis of compounds with high antioxidant and antibacterial activity, such as vanillin, dihydrochalcone-4′-O-glucoside, and 2-hydroxy-3-phenylpropanoic acid.This enhancement leads to increased fruit disease resistance and delayed senescence and decay.

Key words： trypsin; transcriptomics; widely targeted metabolomics; virus-induced gene silencing; induced resistance

Cite this article

SUN Jiaju , WANG Hemin , LI Xin . Trypsin regulates synthesis of phenylpropane compounds induced by CsWRKY33 in mitogen-activated protein kinase pathway of cucumber[J]. Food and Fermentation Industries, 2025 , 51(5) : 95 -102 . DOI: 10.13995/j.cnki.11-1802/ts.039392

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