Cathartic colon is a type of laxative-dependent constipation accompanied by severe enteric nerve and intestinal barrier damage, and probiotic intervention is expected to be an effective treatment for it.In this study, an animal model of the cathartic colon was established using senna extract treatment to study the alleviating effect of Lacticaseibacillus paracasei CCFM1164 on the cathartic colon and to investigate its possible pathways.Results showed that L. paracasei CCFM1164 shortened the time to pass the first black stool and increased the number of fecal pellets within 5 h to relieve constipation in cathartic colon mice.The possible mechanisms underlying the alleviation of the cathartic colon were increasing the relative abundance of short-chain fatty acids (SCFAs)-producing bacteria, such as Lactobacillus and Marvinbryantia, to increase the content of SCFAs in the feces, enhancing the content of acetylcholine (ACh) in the colon, which in turn promotes the secretion of glial cell line-derived neurotrophic factor (GDNF) and thereby repairs the enteric nervous system, and further reducing intestinal inflammation and repairing the intestinal mechanical barrier.This study provides a theoretical basis for probiotics to relieve the cathartic colon.
LU Huimin
,
CHENG Ting
,
ZHANG Tong
,
WANG Linlin
,
WANG Gang
,
ZHAO Jianxin
,
CHEN Wei
. Alleviation of cathartic colon by Lacticaseibacillus paracasei CCFM1164 through modulation of microbiota and intestinal neuroimmune interactions[J]. Food and Fermentation Industries, 2025
, 51(5)
: 111
-118
.
DOI: 10.13995/j.cnki.11-1802/ts.038930
[1] WU J S, LIU B H, TONG W D, et al.Opioid receptors and associated regulator of G protein signaling are involved in the cathartic colon of rats[J].Experimental and Therapeutic Medicine, 2015, 9(4):1229-1234.
[2] 李鑫萍, 王琳琳, 赵建新, 等.两歧双歧杆菌FGSYC45M3缓解泻剂结肠及其作用机制分析[J].食品与发酵工业, 2021, 47(22):42-48.
LI X P, WANG L L, ZHAO J X, et al.Analysis of the effects on cathartic colon alleviation by Bifidobacterium bifidum FGSYC45M3 and its mechanism[J].Food and Fermentation Industries, 2021, 47(22):42-48.
[3] TANG N, YU Q Q, MEI C X, et al.Bifidobacterium bifidum CCFM1163 alleviated cathartic colon by regulating the intestinal barrier and restoring enteric nerves[J].Nutrients, 2023, 15(5):1146.
[4] OBATA Y, CASTAÑO Á, BOEING S, et al.Neuronal programming by microbiota regulates intestinal physiology[J].Nature, 2020, 578(7794):284-289.
[5] SHARKEY K A.Emerging roles for enteric Glia in gastrointestinal disorders[J].The Journal of Clinical Investigation, 2015, 125(3):918-925.
[6] BERGMAN K R, LIU S X L, TIAN R L, et al. Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis[J]. The American Journal of Pathology, 2013, 182(5): 1595-1606.
[7] WANG L L, CHAI M, WANG J L, et al.Bifidobacterium longum relieves constipation by regulating the intestinal barrier of mice[J].Food & Function, 2022, 13(9):5037-5049.
[8] ZHOU X, CHEN Y F, MA X, et al.Efficacy of Bacillus coagulans BC01 on loperamide hydrochloride-induced constipation model in Kunming mice[J].Frontiers in Nutrition, 2022, 9:964257.
[9] 李鑫萍. 乳酸菌对泻剂结肠的缓解作用探究[D].无锡:江南大学, 2021.
LI X P.The study of alleviation effects on cathartic colon by lactic acid bacteria[D].Wuxi:Jiangnan University, 2021.
[10] 毛丙永. 功能性低聚糖对肠道细菌的影响及机制[D].无锡:江南大学, 2015.
MAO B Y.Effects of functional oligosaccharides on the gut bacteria and the mechanism[D].Wuxi:Jiangnan University, 2015.
[11] 张燕, 李红岩.泻剂引起结肠壁形态学改变的临床意义[J].中国临床解剖学杂志, 2004, 22(4):405-407;416.
ZHANG Y, LI H Y.The clinical significance of rhubarb causing the morphological changes in colon wall[J].Chinese Journal of Clinical Anatomy, 2004, 22(4):405-407;416.
[12] KOVLER M L, GONZALEZ SALAZAR A J, FULTON W B, et al.Toll-like receptor 4-mediated enteric Glia loss is critical for the development of necrotizing enterocolitis[J].Science Translational Medicine, 2021, 13(612):eabg3459.
[13] SEGUELLA L, GULBRANSEN B D.Enteric glial biology, intercellular signalling and roles in gastrointestinal disease[J].Nature Reviews.Gastroenterology & Hepatology, 2021, 18(8):571-587.
[14] SORET R, SCHNEIDER S, BERNAS G, et al.Glial cell-derived neurotrophic factor induces enteric neurogenesis and improves colon structure and function in mouse models of hirschsprung disease[J].Gastroenterology, 2020, 159(5):1824-1838.
[15] DE VOS W M, TILG H, VAN HUL M, et al.Gut microbiome and health:Mechanistic insights[J].Gut, 2022, 71(5):1020-1032.
[16] VACCA M, CELANO G, CALABRESE F M, et al.The controversial role of human gut Lachnospiraceae[J].Microorganisms, 2020, 8(4):573.
[17] WU W, ZHOU J W, XUAN R R, et al.Dietary κ-carrageenan facilitates gut microbiota-mediated intestinal inflammation[J].Carbohydrate Polymers, 2022, 277:118830.
[18] YU Z T, LI D G, SUN H X.Herba Origani alleviated DSS-induced ulcerative colitis in mice through remolding gut microbiota to regulate bile acid and short-chain fatty acid metabolisms[J].Biomedicine & Pharmacotherapy, 2023, 161:114409.
[19] YU J S, YOUN G S, CHOI J, et al.Lactobacillus lactis and Pediococcus pentosaceus-driven reprogramming of gut microbiome and metabolome ameliorates the progression of non-alcoholic fatty liver disease[J].Clinical and Translational Medicine, 2021, 11(12):e634.
[20] WANG Z X, SHAO D, WU S, et al.Heat stress-induced intestinal barrier damage and dimethylglycine alleviates via improving the metabolism function of microbiota gut brain axis[J].Ecotoxicology and Environmental Safety, 2022, 244:114053.
[21] YAN C, HUANG S H, DING H F, et al.Adverse effect of oxidized cholesterol exposure on colitis is mediated by modulation of gut microbiota[J].Journal of Hazardous Materials, 2023, 459:132057.
[22] VAN DER HEE B, WELLS J M.Microbial regulation of host physiology by short-chain fatty acids[J].Trends in Microbiology, 2021, 29(8):700-712.
[23] IBIZA S, GARCíA-CASSANI B, RIBEIRO H, et al.Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence[J].Nature, 2016, 535(7612):440-443.
[24] MACPHERSON A J, PACHNIS V, PRINZ M.Boundaries and integration between microbiota, the nervous system, and immunity[J].Immunity, 2023, 56(8):1712-1726.
