Advances in characterization of physically-enzymatically coupled modified soy protein gels

  • YU Mengli ,
  • CHEN Yu ,
  • LIN Fengyan ,
  • CHEN Fusheng
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  • 1(College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China)
    2(Shandong Chemsta Machinery Manufacturing Co.Ltd., Jining 272000, China)
    3(Zhongyuan Food Laboratory, Luohe 462000, China)

Received date: 2024-05-20

  Revised date: 2024-07-05

  Online published: 2025-03-28

Abstract

As a high-quality natural plant protein resource, soybean protein not only contains good nutritional value but also possesses unique functional characteristics.As an important functional property of soybean protein, gelation has attracted much attention, but the natural gel products made from soybean protein have the problems of poor stability and low mechanical strength, which limits the application and development of this kind of gel products.Therefore, it is crucial to improve gel properties of the soybean protein products to meet the diversified needs of the market.In this review, the effects of physical modification, enzyme modification, and physical-enzyme coupling modification on the properties of soybean protein gel were reviewed, the internal relationship between different modification methods and “processing parameters-protein structure-product properties” of soybean protein gel was summarized, and the mechanism behind the effect of physical-enzyme coupling modification on the properties of soybean protein gel was clarified.Hence, this study provides a theoretical basis and technical support for the development of high-quality soybean protein gel products.

Cite this article

YU Mengli , CHEN Yu , LIN Fengyan , CHEN Fusheng . Advances in characterization of physically-enzymatically coupled modified soy protein gels[J]. Food and Fermentation Industries, 2025 , 51(5) : 351 -360 . DOI: 10.13995/j.cnki.11-1802/ts.039942

References

[1] YAO F, CHEN F S, DU Y, et al.Functional and structural properties of soy 11S globulin:Influence of reverse micelle extraction[J].Journal of Food Science, 2021, 86(8):3403-3412.
[2] ZHENG L, REGENSTEIN J M, ZHOU L Y, et al.Soy protein isolates:A review of their composition, aggregation, and gelation[J].Comprehensive Reviews in Food Science and Food Safety, 2022, 21(2):1940-1957.
[3] SHRESTHA S, VAN ’T HAG L, HARITOS V, et al.Rheological and textural properties of heat-induced gels from pulse protein isolates:Lentil, mungbean and yellow pea[J].Food Hydrocolloids, 2023, 143:108904.
[4] BANGAR S P, ESUA O J, SHARMA N, et al.Ultrasound-assisted modification of gelation properties of proteins:A review[J].Journal of Texture Studies, 2022, 53(6):763-774.
[5] 曾剑华, 杨杨, 刘琳琳, 等.热处理过程中大豆11S球蛋白解离缔合行为研究进展[J].食品科学, 2019, 40(11):303-312.
ZENG J H, YANG Y, LIU L L, et al.Recent progress in the dissociation-association behavior of 11S glycinin during heat treatment[J].Food Science, 2019, 40(11):303-312.
[6] FANG L Y, XIANG H, SUN-WATERHOUSE D, et al.Enhancing the usability of pea protein isolate in food applications through modifying its structural and sensory properties via deamidation by glutaminase[J].Journal of Agricultural and Food Chemistry, 2020, 68(6):1691-1697.
[7] LIU Y H, DONG L Z, LI Y, et al.Soy protein isolate-Citrus pectin composite hydrogels induced by TGase and ultrasonic treatment:Potential targeted delivery system for probiotics[J].Food Hydrocolloids, 2023, 143:108901.
[8] LIANG Y R, GUO Y N, ZHENG Y X, et al.Effects of high-pressure homogenization on physicochemical and functional properties of enzymatic hydrolyzed soybean protein concentrate[J].Frontiers in Nutrition, 2022, 9:1054326.
[9] NICOLAI T, CHASSENIEUX C.Heat-induced gelation of plant globulins[J].Current Opinion in Food Science, 2019, 27:18-22.
[10] WU C, MA W C, HUA Y F.The relationship between breaking force and hydrophobic interactions or disulfide bonds involved in heat-induced soy protein gels as affected by heating time and temperature[J].International Journal of Food Science & Technology, 2019, 54(1):231-239.
[11] RUAN Q J, CHEN Y M, KONG X Z, et al.Heat-induced aggregation and sulphydryl/disulphide reaction products of soy protein with different sulphydryl contents[J].Food Chemistry, 2014, 156:14-22.
[12] WU C, WANG T, REN C, et al.Advancement of food-derived mixed protein systems:Interactions, aggregations, and functional properties[J].Comprehensive Reviews in Food Science and Food Safety, 2021, 20(1):627-651.
[13] LV X H, HUANG X, MA B, et al.Modification methods and applications of egg protein gel properties:A review[J].Comprehensive Reviews in Food Science and Food Safety, 2022, 21(3):2233-2252.
[14] ABEDI E, POURMOHAMMADI K.Physical modifications of wheat gluten protein:An extensive review[J].Journal of Food Process Engineering, 2021, 44(3):e13619.
[15] RATHNAKUMAR K, BALAKRISHNAN G, RAMESH B, et al.Impact of emerging food processing technologies on structural and functional modification of proteins in plant-based meat alternatives:An updated review[J].Journal of Texture Studies, 2023, 54(4):599-612.
[16] AKHARUME F U, ALUKO R E, ADEDEJI A A.Modification of plant proteins for improved functionality:A review[J].Comprehensive Reviews in Food Science and Food Safety, 2021, 20(1):198-224.
[17] SULAIMAN N S, SINTANG M D, MOHD ZAINI H, et al.Applications of protein crosslinking in food products[J].International Food Research Journal, 2022, 29(4):723-739.
[18] 陈凤莲, 吉语宁, 贺殷媛, 等.基于凝胶特性的蛋白改性方法研究进展[J].食品研究与开发, 2023, 44(2):190-195.
CHEN F L, JI Y N, HE Y Y, et al.Research progress on protein modification methods based on gel properties[J].Food Research and Development, 2023, 44(2):190-195.
[19] WANG J M, NA X K, NAVICHA W B, et al.Concentration-dependent improvement of gelling ability of soy proteins by preheating or ultrasound treatment[J].LWT, 2020, 134:110170.
[20] MALAKI NIK A, TOSH S M, WOODROW L, et al.Effect of soy protein subunit composition and processing conditions on stability and particle size distribution of soymilk[J].LWT-Food Science and Technology, 2009, 42(7):1245-1252.
[21] LI X F, CHEN L Y, HUA Y F, et al.Effect of preheating-induced denaturation during protein production on the structure and gelling properties of soybean proteins[J].Food Hydrocolloids, 2020, 105:105846.
[22] 武雅琴. 改性大豆蛋白与鱼类肌原纤维蛋白的相互作用及复合凝胶特性[D].上海:上海海洋大学, 2019.
WU Y Q.Interaction between modified soybean protein and fish myofibrillar protein and characteristics of composite gel[D].Shanghai:Shanghai Ocean University, 2019.
[23] FLOURY J, DESRUMAUX A, LEGRAND J.Effect of ultra-high-pressure homogenization on structure and on rheological properties of soy protein-stabilized emulsions[J].Journal of Food Science, 2002, 67(9):3388-3395.
[24] KEERATI-U-RAI M, CORREDIG M.Effect of dynamic high pressure homogenization on the aggregation state of soy protein[J].Journal of Agricultural and Food Chemistry, 2009, 57(9):3556-3562.
[25] KANG Z L, BAI R, LU F, et al.Effects of high pressure homogenization on the solubility, foaming, and gel properties of soy 11S globulin[J].Food Hydrocolloids, 2022, 124:107261.
[26] KANG Z L, ZHANG X H, LI X, et al.The effects of sodium chloride on proteins aggregation, conformation and gel properties of pork myofibrillar protein Running Head:Relationship aggregation, conformation and gel properties[J].Journal of Food Science and Technology, 2021, 58(6):2258-2264.
[27] SUN D Y, WU M, BI C H, et al.Using high-pressure homogenization as a potential method to pretreat soybean protein isolate:Effect on conformation changes and rheological properties of its acid-induced gel[J].Innovative Food Science & Emerging Technologies, 2022, 82:103195.
[28] ZHENG L, WANG Z J, KONG Y, et al.Different commercial soy protein isolates and the characteristics of Chiba tofu[J].Food Hydrocolloids, 2021, 110:106115.
[29] GHARIBZAHEDI S M T, SMITH B.The functional modification of legume proteins by ultrasonication:A review[J].Trends in Food Science & Technology, 2020, 98:107-116.
[30] ZHENG T, LI X H, TAHA A, et al.Effect of high intensity ultrasound on the structure and physicochemical properties of soy protein isolates produced by different denaturation methods[J].Food Hydrocolloids, 2019, 97:105216.
[31] HU H, FAN X, ZHOU Z, et al.Acid-induced gelation behavior of soybean protein isolate with high intensity ultrasonic pre-treatments[J].Ultrasonics Sonochemistry, 2013, 20(1):187-195.
[32] 刘冉, 曾庆华, 王振宇, 等.超声波处理对大豆分离蛋白凝胶流变性和凝胶形成的影响[J].食品工业科技, 2020, 41(21):87-92; 98.
LIU R, ZENG Q H, WANG Z Y, et al.Effects of ultrasonic treatment on gel rheological properties and gel formation of soybean protein isolate[J].Science and Technology of Food Industry, 2020, 41(21):87-92; 98.
[33] ZOU Y, YANG H, LI P P, et al.Effect of different time of ultrasound treatment on physicochemical, thermal, and antioxidant properties of chicken plasma protein[J].Poultry Science, 2019, 98(4):1925-1933.
[34] AFKHAMI R, VARIDI M J, VARIDI M, et al.Improvement of heat-induced nanofibrils formation of soy protein isolate through NaCl and microwave[J].Food Hydrocolloids, 2023, 139:108443.
[35] LI Y Y, WANG S N, LIU X L, et al.Improvement in texture and color of soy protein isolate gel containing capsorubin and carotenoid emulsions following microwave heating[J].Food Chemistry, 2023, 428:136743.
[36] BHATTACHARYA S, JENA R.Gelling behavior of defatted soybean flour dispersions due to microwave treatment:Textural, oscillatory, microstructural and sensory properties[J].Journal of Food Engineering, 2007, 78(4):1305-1314.
[37] QIN X S, LUO S Z, CAI J, et al.Effects of microwave pretreatment and transglutaminase crosslinking on the gelation properties of soybean protein isolate and wheat gluten mixtures[J].Journal of the Science of Food and Agriculture, 2016, 96(10):3559-3566.
[38] LIU H H, KUO M I.Effect of microwave heating on the viscoelastic property and microstructure of soy protein isolate gel[J].Journal of Texture Studies, 2011, 42(1):1-9.
[39] CHEN Y, LIANG Y, LIU H, et al.Extrusion of wheat gluten-peanut oil complexes and their rheological characteristics[J].Food Chemistry, 2021, 364:130435.
[40] CHEN F L, WEI Y M, ZHANG B.Chemical cross-linking and molecular aggregation of soybean protein during extrusion cooking at low and high moisture content[J].LWT-Food Science and Technology, 2011, 44(4):957-962.
[41] SINGH R, KOKSEL F.Effects of particle size distribution and processing conditions on the techno-functional properties of extruded soybean meal[J].LWT, 2021, 152:112321.
[42] ZHANG S T, HUANG W J, ROOPESH M S, et al.Pre-treatment by combining atmospheric cold plasma and pH-shifting to prepare pea protein concentrate powders with improved gelling properties[J].Food Research International, 2022, 154:111028.
[43] 王然, 龚维, 郭晓璐, 等.冷等离子体处理改性大豆分离蛋白及改善其界面性能[J].现代食品科技, 2022, 38(12):101-109.
WANG R, GONG W, GUO X L, et al.Modification of soybean protein isolate by cold plasma treatment and improvement of its interface properties[J].Modern Food Science and Technology, 2022, 38(12):101-109.
[44] MIWA N.Innovation in the food industry using microbial transglutaminase:Keys to success and future prospects[J].Analytical Biochemistry, 2020, 597:113638.
[45] CHEN D, CAMPANELLA O H.Limited enzymatic hydrolysis induced pea protein gelation at low protein concentration with less heat requirement[J].Food Hydrocolloids, 2022, 128:107547.
[46] LI C Y, WU X F, MU D D, et al.Effect of partial hydrolysis with papain on the characteristics of transglutaminase-crosslinked tofu gel[J].Journal of Food Science, 2018, 83(12):3092-3098.
[47] YANG J J, ZHU B, DOU J J, et al.Structural characterization of soy protein hydrolysates and their transglutaminase-induced gelation properties[J].LWT, 2023, 179:114668.
[48] LOPES-DA-SILVA J A, MONTEIRO S R.Gelling and emulsifying properties of soy protein hydrolysates in the presence of a neutral polysaccharide[J].Food Chemistry, 2019, 294:216-223.
[49] 郑环宇, 赵晓明, 张梦, 等.不同酶切方式引发大豆蛋白构象变化及功能特性评价[J].中国粮油学报, 2022, 37(6):103-111.
ZHENG H Y, ZHAO X M, ZHANG M, et al.Evaluation on changes and functional properties of soy protein conformation due to different enzymolysis methods[J].Journal of the Chinese Cereals and Oils Association, 2022, 37(6):103-111.
[50] WANG C Y, LI T Q, MA L, et al.Consequences of superfine grinding treatment on structure, physicochemical and rheological properties of transglutaminase-crosslinked whey protein isolate[J].Food Chemistry, 2020, 309:125757.
[51] FATIMA S W, KHARE S K.Current insight and futuristic vistas of microbial transglutaminase in nutraceutical industry[J].Microbiological Research, 2018, 215:7-14.
[52] LIU Y X, ZHANG Y F, GUO Z H, et al.Enhancing the functional characteristics of soy protein isolate via cross-linking catalyzed by Bacillus subtilis transglutaminase[J].Journal of the Science of Food and Agriculture, 2021, 101(10):4154-4160.
[53] LI Y Y, WANG S N, LIU X L, et al.Characterization of enzymatic cross-linking soy protein isolate xerogels and its shape memory effect induced by ethylcellulose[J].Food Chemistry, 2023, 412:135564.
[54] MI H B, ZHAO Y M, LI Y, et al.Combining effect of soybean protein isolate and transglutaminase on the gel properties of Zhikong scallop (Chlamys Farreri) adductor muscle[J].LWT, 2021, 138:110727.
[55] LV Y Q, TANG T T, XU L L, et al.Influence of soybean dietary fiber with varying particle sizes and transglutaminase on soy protein isolate gel[J].Food Research International, 2022, 161:111876.
[56] GAN J, SUN L R, GUAN C X, et al.Preparation and properties of salecan-soy protein isolate composite hydrogel induced by thermal treatment and transglutaminase[J].International Journal of Molecular Sciences, 2022, 23(16):9383.
[57] XU J J, GUO S Y, LI X J, et al.Gel properties of transglutaminase-induced soy protein isolate-polyphenol complex:Influence of epigallocatechin-3-gallate[J].Journal of the Science of Food and Agriculture, 2021, 101(9):3870-3879.
[58] QIN X S, LUO S Z, CAI J, et al.Transglutaminase-induced gelation properties of soy protein isolate and wheat gluten mixtures with high intensity ultrasonic pretreatment[J].Ultrasonics Sonochemistry, 2016, 31:590-597.
[59] ZHAO L, CHEN M H, BI X Z, et al.Physicochemical properties, structural characteristics and in vitro digestion of brown rice-pea protein isolate blend treated by microbial transglutaminase[J].Food Hydrocolloids, 2023, 141:108673.
[60] LIANG G J, CHEN W P, QIE X J, et al.Modification of soy protein isolates using combined pre-heat treatment and controlled enzymatic hydrolysis for improving foaming properties[J].Food Hydrocolloids, 2020, 105:105764.
[61] LIU Y Q, HUANG Y Y, DENG X Q, et al.Effect of enzymatic hydrolysis followed after extrusion pretreatment on the structure and emulsibility of soybean protein[J].Process Biochemistry, 2022, 116:173-184.
[62] 李杨, 马召蕾, 郑丽, 等.超声联合酶处理下TG酶交联大豆分离蛋白凝胶特性研究[J].农业机械学报, 2022, 53(4):394-402.
LI Y, MA Z L, ZHENG L, et al.Effect of ultrasonic combined with enzyme treatment on glutamine transaminase cross-linked soybean protein isolate gels[J].Transactions of the Chinese Society for Agricultural Machinery, 2022, 53(4):394-402.
[63] WU X X, GAO T, XU Z, et al.Effect of combined enzyme and ultrasound treatment on the structure and gel properties of soy protein isolate:A comparative study of alkaline protease and pepsin[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects, 2024, 687:133533.
[64] ZHANG P P, HU T, FENG S L, et al.Effect of high intensity ultrasound on transglutaminase-catalyzed soy protein isolate cold set gel[J].Ultrasonics Sonochemistry, 2016, 29:380-387.
[65] MA Z L, LI L J, WU C L, et al.Effects of combined enzymatic and ultrasonic treatments on the structure and gel properties of soybean protein isolate[J].LWT, 2022, 158:113123.
[66] MAO Y X, ZHAO C B, QI Q, et al.Transglutaminase-induced soybean protein isolate cold-set gels treated with combination of ultrasound and high pressure:Physicochemical properties and structural characterization[J].International Journal of Biological Macromolecules, 2023, 253:127525.
[67] WANG X F, WANG Y Q, WANG X S, et al.Influence of substrate aggregation state on the enzymatic-induced crosslinking of soy protein isolate[J].Food Chemistry, 2024, 442:138484.
[68] ZHANG L, ZHANG J X, WEN P P, et al.Effect of high-intensity ultrasound pretreatment on the properties of the transglutaminase (TGase)-induced β-conglycinin (7S) gel[J].Foods, 2023, 12(10):2037.
[69] ZHANG P, BAO Z Y, WANG H, et al.Ultrasonic pretreatment improved the physicochemical properties and riboflavin delivery ability of transglutaminase-catalyzed soy protein isolate gel[J].Food Hydrocolloids, 2022, 131:107782.
[70] QIN X S, CHEN S S, LI X J, et al.Gelation properties of transglutaminase-induced soy protein isolate and wheat gluten mixture with ultrahigh pressure pretreatment[J].Food and Bioprocess Technology, 2017, 10(5):866-874.
[71] 史乾坤, 王心雅, 甄诺, 等.超高压预处理对TGase交联的大豆分离蛋白凝胶的影响[J].中国粮油学报, 2021, 36(9):94-100.
SHI Q K, WANG X Y, ZHEN N, et al.Effects of ultra high pressure pretreatment on TGase cross-linked soy protein isolate gel[J].Journal of the Chinese Cereals and Oils Association, 2021, 36(9):94-100.
[72] ZHANG M Q, YANG Y J, ACEVEDO N C.Effects of pre-heating soybean protein isolate and transglutaminase treatments on the properties of egg-soybean protein isolate composite gels[J].Food Chemistry, 2020, 318:126421.
[73] SUN P, ZHANG Q, ZHAO Y, et al.Improving gel properties of soy protein isolate through alkaline pH-shifting, mild heat treatment, and TGase cross-linking[J].Food Hydrocolloids, 2023, 144:108924.
[74] MU D D, LI H W, LI X J, et al.Enhancing laccase-induced soybean protein isolates gel properties by microwave pretreatment[J].Journal of Food Processing and Preservation, 2020, 44(4):e14386.
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